Comparative analysis of the efficiency of intron-length polymorphism of β-tubulin genes and microsatellite loci for flax varieties genotyping

Resumo

Efficacy of the β-tubulin introns lenght polymorphism method (TBP) was used for Ukrainian bredeed flax cultivars genotyping. For this purpose, TBP data were compared with data produced using the two most effective species-specific SSR markers. Both methods were used to evaluate intra- and intercultivar flax polymorphism. For each cultivar, PIC data (Polymorphism Information Content) and the range of allele lenghts, as well as the number of allele phenotypes, were calculated using TBP and SSR markers. The dendrograms, built using Nei and Li’s similarity coefficient, differ for SSR and TBP markers. Most flax cultivars of Ukrainian selection were genetically heterogeneous. The TBP method was highly efficient for differentiation of flax genotypes versus SSR analysis.