Enhancement of Agrobacterium-mediated transformation of plants using trifluoperazine and genistein—protein kinase inhibitors


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Аннотация

The effect of trifluoperazine, the inhibitor of serine/threonine protein kinases, and genistein, the inhibitor of tyrosine protein kinases, on the frequency of Agrobacterium-mediated transformation of angiosperms has been studied on the example of tobacco as a model plant. The study of the effects produced by trifluoperazine in a wide range of concentrations from 10 to 300 μM has allowed us to establish that the use of trifluoperazine at the concentration of 10 μM increases the frequency of Agrobacterium-mediated transformation of tobacco leaf discs by 25%. The effect of genistein also in a wide range of concentrations from 10 to 100 μM was studied in parallel and its most efficient concentration (100 μM) has been found for Agrobacterium-mediated transformation of tobacco leaf discs, at which the frequency of transformation increased by 12%.

Авторлар туралы

A. Yemets

Institute of Food Biotechnology and Genomics

Хат алмасуға жауапты Автор.
Email: yemets.alla@gmail.com
Украина, Kyiv

V. Fedorchuk

Institute of Food Biotechnology and Genomics

Email: yemets.alla@gmail.com
Украина, Kyiv

Y. Blume

Institute of Food Biotechnology and Genomics

Email: yemets.alla@gmail.com
Украина, Kyiv

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© Allerton Press, Inc., 2016