Verification of Grape Pedigree by Microsatellite Analysis

Abstract

Microsatellite DNA markers were used to identify and compare the genotypes of 80 samples of grapes and their presumptive parental forms. Both presumptive parental forms were confirmed for 51 samples. Further statistical evaluation showed that the probability of descent from particularly the pair of parents identified at the previous stage (X × Y) was higher than the probability of descent from any random varieties not included in the study. The X × Y index ranged from 10³ to 10¹². The identity of one of the parents was not confirmed for four samples due to nonmatching allele profiles of the microsatellite loci studied. Genotypes of the ancestral forms were used in pedigree analysis of 22 samples from the set, because plants of the presumptive parental forms were physically unavailable and there was no information on the composition of their microsatellite profiles.