Genotyping of Triticum ssp. Hexaploid Species Samples with ISSR-Markers
- Authors: Kyrienko A.V.1,2, Rozhkov R.V.2, Parii M.F.2, Symonenko Y.V.1,2
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Affiliations:
- Institute of Cell Biology and Genetic Engineering
- All-Ukrainian Scientific Institute of Breeding
- Issue: Vol 52, No 4 (2018)
- Pages: 276-282
- Section: Article
- URL: https://journals.rcsi.science/0095-4527/article/view/173945
- DOI: https://doi.org/10.3103/S0095452718040047
- ID: 173945
Cite item
Abstract
The genetic improvement of soft wheat (Triticum aestivum L.) and other hexaploid wheat species (T. spelta, T. spherococcum, T. petropavlovskyi, and T. compactum) is important. This can be done by transferring genes of interest (resistance to biotic and abiotic factors). For this purpose, phylogenetic connections of the studied genotypes should be investigated. This problem can be solved by using a multilocus system based on ISSR markers. The marker system is highly polymorphic and convenient for analysis. The study presents the results of a comparison of 20 different hexaploid wheat genotypes based on ISSR markers. In addition, the level of polymorphism was determined and a dendrogram reflecting the phylogenetic connections between the studied genotypes was constructed. It was shown that the species located next to each other according to the systematics turned out to be more remote after the ISSR-marker analysis, and, vice versa, the species that were systematically more distant showed a higher level of kinship.
About the authors
A. V. Kyrienko
Institute of Cell Biology and Genetic Engineering; All-Ukrainian Scientific Institute of Breeding
Author for correspondence.
Email: anastasija.kirienko@gmail.com
Ukraine, Kyiv, 03680; Kyiv, 03022
R. V. Rozhkov
All-Ukrainian Scientific Institute of Breeding
Email: anastasija.kirienko@gmail.com
Ukraine, Kyiv, 03022
M. F. Parii
All-Ukrainian Scientific Institute of Breeding
Email: anastasija.kirienko@gmail.com
Ukraine, Kyiv, 03022
Yu. V. Symonenko
Institute of Cell Biology and Genetic Engineering; All-Ukrainian Scientific Institute of Breeding
Email: anastasija.kirienko@gmail.com
Ukraine, Kyiv, 03680; Kyiv, 03022