Karyotypic study of five Lutjanid species using conventional and Ag-NORs banding techniques
- Authors: Phimphan S.1, Supiwong W.2, Tanomtong A.1, Pinthong K.3, Sangpakdee W.4, Kaewsri S.5
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Affiliations:
- Department of Biology, Faculty of Science
- Faculty of Applied Science and Engineering
- Department of Fundamental Science, Faculty of Science and Technology
- Biology Program, Faculty of Science
- Biology Program, Department of Science, Faculty of Science
- Issue: Vol 51, No 4 (2017)
- Pages: 315-324
- Section: Article
- URL: https://journals.rcsi.science/0095-4527/article/view/173688
- DOI: https://doi.org/10.3103/S0095452717040077
- ID: 173688
Cite item
Abstract
The first cytogenetic comparisons of five snapper species from Thailand were presented here. Renal cell samples were taken from blacktail snapper (Lutjanus fulvus), five lined snapper (L. quinquelineatus), dory snapper (L. fulviflamma), brownstripe red snapper (L. vitta), and mangrove red snapper (L. argentimaculatus). The mitotic chromosome preparation was prepared directly from kidney cells. Conventional staining and Ag-NOR banding techniques were applied to stain the chromosomes. The results exhibited that all five snapper species have the diploid chromosome numbers of 2n = 48 and the fundamental numbers (NF) of 48. The presences of large, medium, and small telocentric chromosomes were 22-24-2, 24-20-4, 36-10-2, 28-16-4 and 36-10-2, respectively. The Ag- NORs banding technique provides the pair of nucleolar organizer regions (NORs) at subcentromeric region of the long arm of the respective telocentric chromosome pairs 9, 1, 3, 4 and 9. Their karyotype formulas is as follows: L. fulvus (2n = 48): L22t + M24t + S2t, L. quinquelineatus (2n = 48): L24t + M20t + S4t, L. fulviflamma (2n = 48): Lt36 + Mt10 + St2, L. vitta (2n = 48): L28t + M16t + S4t, and L. argentimaculatus (2n = 48): L36t + M10t + S2t.
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About the authors
Sumalee Phimphan
Department of Biology, Faculty of Science
Email: tanomtong@hotmail.com
Thailand, Muang, Khon Kaen, 40002
Weerayuth Supiwong
Faculty of Applied Science and Engineering
Email: tanomtong@hotmail.com
Thailand, Muang, Nongkhai, 43000
Alongklod Tanomtong
Department of Biology, Faculty of Science
Author for correspondence.
Email: tanomtong@hotmail.com
Thailand, Muang, Khon Kaen, 40002
Krit Pinthong
Department of Fundamental Science, Faculty of Science and Technology
Email: tanomtong@hotmail.com
Thailand, Muang, Surin, 32000
Wiwat Sangpakdee
Biology Program, Faculty of Science
Email: tanomtong@hotmail.com
Thailand, Muang, Udon Thani, 41000
Sarawut Kaewsri
Biology Program, Department of Science, Faculty of Science
Email: tanomtong@hotmail.com
Thailand, Muang, Buriram, 31000