Histological peculiarities of Triticum aestivum L. calli cultures’ morphogenesis under antibiotic Ceftriaxone influence

详细

It was shown that, for efficient production of regenerants in bread wheat, 18-day-old calli obtained from apical meristems of 3-day-old aseptic seedlings should be used. These calli show proliferative centers, with developed vascular system elements, that support the development of meristematic centers that later produce regenerants. It was shown that regeneration from the calli occurs by direct organogenesis but not by somatic embryogenesis. Under the influence of the antibiotic Ceftriaxone, which was added to the regeneration medium in a concentration of 400 mg/L, two different bread wheat genotypes showed, firstly, changes in apical origin callus morphology; secondly, an intensification of the regeneration processes; thirdly, root development in the regenerants that allows one to avoid the additional step of plant rooting.