Phenolic Compounds of Codiaeum variegatum Spirale Lessened Cytotoxic and Genotoxic Effects of Mitomycin C in Mice Somatic and Germ Cells
- 作者: Mona A.M. Abo-Zeid 1, Farghaly A.1, Hassan E.2, Abdel-Samie N.1
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隶属关系:
- Genetics and Cytology Department, Genetic Engineering and Biotechnology Research Division, National Research Centre
- Medicinal and Aromatic Plants Research Department, Pharmaceutical and Drug Industries Research division, National Research Centre
- 期: 卷 53, 编号 6 (2019)
- 页面: 494-501
- 栏目: Article
- URL: https://journals.rcsi.science/0095-4527/article/view/174173
- DOI: https://doi.org/10.3103/S0095452719060057
- ID: 174173
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详细
This study evaluates the chemopreventive properties of phenolic compounds extracted from Codiaeum variegatum (PCCV) against cytotoxic and genotoxic effects of mitomycin C (MMC). Male Swiss albino mice were treated with PCCV and/or MMC, and samples were collected 24 h after the last treatments. We recorded chromosome aberrations in bone-marrow cells and spermatocytes, cell viability and DNA damage (using comet assay) in bone-marrow cells. We observed that the highest concentration of PCCV did not induce cytotoxic or genotoxic effects on mice somatic and germ cells. However, MMC reduced cell proliferation remarkably (p < 0.05), and increased chromosome aberrations and DNA damage significantly. Additionally, PCCV prohibited cytotoxicity and genotoxicity of MMC when administered to animals 2h prior to MMC. Evidently, PCCV induced a significant inhibition (p < 0.05) in the percentages of chromosome aberrations in bone-marrow cells excluding gaps (19.82, 25.23, and 42.34%) and spermatocytes (22.73, 31.82, and 48.48%) at concentrations 125, 250 and 500 mg/kg b.wt respectively. Likewise, PCCV declined tail DNA (%), tail length (μm) and tail moment, and the inhibitory index of tail DNA (%) reached to 89.44% at 500 mg/kg b.wt. In conclusion, phenolic compounds of Codiaeum variegatum reduced the cytotoxic and genotoxic effects that were induced by the mutagenic agent MMC.
作者简介
Mona A.M. Abo-Zeid
Genetics and Cytology Department, Genetic Engineering and Biotechnology Research Division,National Research Centre
编辑信件的主要联系方式.
Email: monaabozeid@yahoo.com
埃及, Cairo, Dokki, 12622
Ayman Farghaly
Genetics and Cytology Department, Genetic Engineering and Biotechnology Research Division,National Research Centre
Email: monaabozeid@yahoo.com
埃及, Cairo, Dokki, 12622
Emad Hassan
Medicinal and Aromatic Plants Research Department, Pharmaceutical and Drug Industries Research division,National Research Centre
Email: monaabozeid@yahoo.com
埃及, Cairo, Dokki, 12622
Negm Abdel-Samie
Genetics and Cytology Department, Genetic Engineering and Biotechnology Research Division,National Research Centre
Email: monaabozeid@yahoo.com
埃及, Cairo, Dokki, 12622