The Influence of Recombinant Interferon α2b Synthesized in Plants on the Reparative Enzyme MGMT Expression in Human Somatic Cells in vitro
- Authors: Nidoieva Z.M.1, Peterson A.A.2, Ruban T.P.1, Dzuba G.V.1,3, Kuchuk M.V.2, Lukash L.L.1
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Affiliations:
- Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine
- Institute of Cell Biology and Genetic Engineering
- ESC Institute of Biology and Medicine of Taras Shevchenko Kyiv National University
- Issue: Vol 53, No 6 (2019)
- Pages: 467-472
- Section: Article
- URL: https://journals.rcsi.science/0095-4527/article/view/174162
- DOI: https://doi.org/10.3103/S0095452719060070
- ID: 174162
Cite item
Abstract
We investigated the influence of transgene interferon α2β synthesized in plants on human repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) in both cancer and non-cancer originated cells. Using Western-blot analysis we determined MGMT quantity in cancer-originated Hеp-2 cells (epidermoid carcinoma of the larynx) and non-cancer E8 ones (derived in our laboratory from germline cells) were treated with purified transgene interferon α2β in serum-free medium. Interferon α2β caused decrease of the MGMT protein amount in the cancer-originated Hеp-2 cells at all treated concentrations (2, 20, 200, 2000 IU/mL) relative to the control. In human non-cancer originated E8 cells we revealed a decrease in MGMT levels at the both highest concentrations: 200 and 2000 IU/mL, although only at 200 IU/mL the downregulation effect was statistically significant. Thus, the transgenic interferon inhibitory effect was more noticeable in the cancer-originated cells compared to the non-cancer ones.
About the authors
Z. M. Nidoieva
Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine
Author for correspondence.
Email: zarinanidoeva@i.ua
Ukraine, Kyiv, 03143
A. A. Peterson
Institute of Cell Biology and Genetic Engineering
Email: lukash@imbg.org.ua
Ukraine, Kyiv, 03143
T. P. Ruban
Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine
Email: lukash@imbg.org.ua
Ukraine, Kyiv, 03143
G. V. Dzuba
Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine; ESC Institute of Biology and Medicine of Taras Shevchenko Kyiv National University
Email: lukash@imbg.org.ua
Ukraine, Kyiv, 03143; Kyiv, 03127
M. V. Kuchuk
Institute of Cell Biology and Genetic Engineering
Author for correspondence.
Email: nkuchuk@icbge.org.ua
Ukraine, Kyiv, 03143
L. L. Lukash
Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine
Author for correspondence.
Email: lukash@imbg.org.ua
Ukraine, Kyiv, 03143