Isolation, Characterization and Association among Phosphate Solubilizing Bacteria from Sugarcane Rhizosphere

Abstract

One of the premium qualities of Phosphate solubilizing bacteria is to solubilize insoluble phosphorus to make it available for plant roots to be engrossed. To check the ability for phosphate solubilization, production of indole acetic acid, antagonistic activity against fungal pathogen and intrinsic antibiotic resistance phosphorous solubilizing bacterial isolates were isolated and screened. In total, 12 PSB were found rod shaped cells being gram negative. Different levels of antibiotic resistance were observed by rhizobacterial isolates against four antibiotics (Ampicillin, Kanamycin, Tetracycline and Streptomycin 25, 30, 30 and 10 μg/mL respectively). The isolates S7 and S20 showed antifungal activity against Fusarium oxysporum. Conversion of insoluble phosphorous Ca₃(PO₄) into IAA was observed by all PSB Isolates. Two phosphorous solubilizing bacterial isolates sequences were submitted in NCBI database. Conclusively, good antifungal activity with greater ability to solubilize insoluble phosphorus can be achieved by combine application of rhizobacterial isolates with S22. Further, it is an eco-friendly and cost effective strategy to improve crop production.