Peculiarities of Regeneration and Genetic Variability of Crambe koktebelica and Crambe tataria Plants in vitro
- Authors: Pushkarova N.O.1, Lakhneko O.R.1, Belokurova V.B.1, Morgun B.V.1, Kuchuk M.V.1
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Affiliations:
- Institute of Cell Biology and Genetic Engineering
- Issue: Vol 52, No 4 (2018)
- Pages: 269-275
- Section: Article
- URL: https://journals.rcsi.science/0095-4527/article/view/173941
- DOI: https://doi.org/10.3103/S0095452718040096
- ID: 173941
Cite item
Abstract
The regenerative capability of three types of explants was studied on media with different compositions of growth regulators with the purpose of selecting optimal conditions of fast reproduction of endangered Crambe species that could be used as a relevant source of genetic material for the improvement of industrially valuable plants. PCR-analysis of genotypes of C. koktebelica and C. tataria plants was conducted to identify the influence of in vitro cultivation on the genetic stability of plants. The highest regeneration rates were observed with the use of petiole explants on MS medium with BA and NAA. The absence of somaclonal variability in C. koktebelica and C. tataria in vitro regenerated plants was demonstrated.
About the authors
N. O. Pushkarova
Institute of Cell Biology and Genetic Engineering
Author for correspondence.
Email: nadu4ka@gmail.com
Ukraine, Kyiv
O. R. Lakhneko
Institute of Cell Biology and Genetic Engineering
Email: nadu4ka@gmail.com
Ukraine, Kyiv
V. B. Belokurova
Institute of Cell Biology and Genetic Engineering
Email: nadu4ka@gmail.com
Ukraine, Kyiv
B. V. Morgun
Institute of Cell Biology and Genetic Engineering
Email: nadu4ka@gmail.com
Ukraine, Kyiv
M. V. Kuchuk
Institute of Cell Biology and Genetic Engineering
Email: nadu4ka@gmail.com
Ukraine, Kyiv