Influence of different concentrations of ascorbic and gibberellic acids and pH of medium on embryogenesis and regeneration in anther culture of spring triticale
- Authors: Yerzhebayeva R.S.1, Abekova A.M.1, Ainebekova B.A.1, Urazaliyev K.R.1, Bazylova T.A.1, Daniyarova A.K.1, Bersimbayeva G.K.1
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Affiliations:
- Kazakh Research Institute of Agriculture and Plant Growing
- Issue: Vol 51, No 6 (2017)
- Pages: 448-454
- Section: Article
- URL: https://journals.rcsi.science/0095-4527/article/view/173762
- DOI: https://doi.org/10.3103/S0095452717060032
- ID: 173762
Cite item
Abstract
In this work, the effect of ascorbic and gibberellic acids and pH of medium on embryogenesis and regeneration in anther culture of spring triticale was studied. The study of androgenesis was conducted on two model samples YaTKh-327-11 and YaTKh-18-11. The research revealed that the addition of the antioxidant ascorbic acid at a concentration of 2–8 mg/L into the culture medium to induce embryogenesis enhanced the formation of androgen structures and regeneration of green plants. The addition of gibberellic acid into the culture medium to induce embryogenesis gave negative effects. The average yield of green plants in the anther culture of spring triticale was 2 pcs/100 anthers. According to the research, 277 green plants were received and spontaneous doubling of chromosomes was recorded in 26.7%.
About the authors
R. S. Yerzhebayeva
Kazakh Research Institute of Agriculture and Plant Growing
Author for correspondence.
Email: raushan_2008@mail.ru
Kazakhstan, Almalybak Village, Karasay district, Almaty oblast
A. M. Abekova
Kazakh Research Institute of Agriculture and Plant Growing
Email: raushan_2008@mail.ru
Kazakhstan, Almalybak Village, Karasay district, Almaty oblast
B. A. Ainebekova
Kazakh Research Institute of Agriculture and Plant Growing
Email: raushan_2008@mail.ru
Kazakhstan, Almalybak Village, Karasay district, Almaty oblast
K. R. Urazaliyev
Kazakh Research Institute of Agriculture and Plant Growing
Email: raushan_2008@mail.ru
Kazakhstan, Almalybak Village, Karasay district, Almaty oblast
T. A. Bazylova
Kazakh Research Institute of Agriculture and Plant Growing
Email: raushan_2008@mail.ru
Kazakhstan, Almalybak Village, Karasay district, Almaty oblast
A. K. Daniyarova
Kazakh Research Institute of Agriculture and Plant Growing
Email: raushan_2008@mail.ru
Kazakhstan, Almalybak Village, Karasay district, Almaty oblast
G. Kh. Bersimbayeva
Kazakh Research Institute of Agriculture and Plant Growing
Email: raushan_2008@mail.ru
Kazakhstan, Almalybak Village, Karasay district, Almaty oblast