Cryosynthesis and Properties of Dehydroepiandrosterone Hormone Nanoparticles
- 作者: Morozov Y.1, Chistyakov D.2, Utekhina A.1, Astakhova A.2, Goncharov N.3, Sergeeva M.2, Sergeev G.1
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隶属关系:
- Department of Chemistry, M. V. Lomonosov Moscow State University
- A. N. Belozerskii Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University
- Endocrinology Research Center, Russian Federation Ministry of Health
- 期: 卷 49, 编号 12 (2016)
- 页面: 813-816
- 栏目: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/244298
- DOI: https://doi.org/10.1007/s11094-016-1377-2
- ID: 244298
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详细
Nanoparticles of the steroidal hormone dihydroepiandrosterone (DHEA) were prepared by cryosynthesis technology, which allowed starting DHEA particles of size (100 ± 50) μm to be converted to nanoparticles of size (100 ± 20) nm. The particle sizes were determined by optical, electron transmission, and scanning atomic-force microscopy. HPLC with mass-spectrometric detection of starting and modified DHEA samples showed that the hormone molecular structure did not change during cryosynthesis of the nanoparticles. A comparison of the cytotoxicities of the samples on C6 glial cell culture showed that modified DHEA was less toxic. The results indicated that cryosynthesis technology could be used effectively to prepare nanoparticles of steroidal hormones.
作者简介
Yu. Morozov
Department of Chemistry, M. V. Lomonosov Moscow State University
Email: chem@folium.ru
俄罗斯联邦, Moscow, 119991
D. Chistyakov
A. N. Belozerskii Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University
Email: chem@folium.ru
俄罗斯联邦, Moscow, 119234
A. Utekhina
Department of Chemistry, M. V. Lomonosov Moscow State University
Email: chem@folium.ru
俄罗斯联邦, Moscow, 119991
A. Astakhova
A. N. Belozerskii Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University
Email: chem@folium.ru
俄罗斯联邦, Moscow, 119234
N. Goncharov
Endocrinology Research Center, Russian Federation Ministry of Health
Email: chem@folium.ru
俄罗斯联邦, Moscow, 117036
M. Sergeeva
A. N. Belozerskii Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University
Email: chem@folium.ru
俄罗斯联邦, Moscow, 119234
G. Sergeev
Department of Chemistry, M. V. Lomonosov Moscow State University
Email: chem@folium.ru
俄罗斯联邦, Moscow, 119991