Analytical Method Development and Validation for HPLC-ECD Determination of Moxifloxacin in Marketed Formulations
- Авторы: Phani Sekhar Reddy G.1, Navyasree K.1, Jagadish P.1, Bhat K.1
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Учреждения:
- Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
- Выпуск: Том 52, № 7 (2018)
- Страницы: 674-679
- Раздел: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/245400
- DOI: https://doi.org/10.1007/s11094-018-1879-1
- ID: 245400
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Аннотация
An HPLC-ECD analytical method with high reproducibility and wide linearity range has been developed and validated. Moxifloxacin was separated and identified using this method with a simple mobile phase comprising Britton Robinson buffer pH 5.0 and methanol (93: 7 v/v) flowing at a rate of 0.5 mL/min through Acclaim C18 column (150 mm × 4.6 mm × 5 im) maintained at 35°C and detected at redox potential value of 1.0 V. The LOD and LOQ were found to be 2.2 and 6.6 μg/mL, respectively. The HPLC-ECD method of moxifloxacin analysis was validated as per ICH Q2R1 guidelines and applied for assay of marketed moxifloxacin formulations to establish the acceptable recovery.
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Об авторах
G. Phani Sekhar Reddy
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
Email: km.bhat@manipal.edu
Индия, Madhav Nagar, Manipal, Karnataka, 576104
K. Navyasree
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
Email: km.bhat@manipal.edu
Индия, Madhav Nagar, Manipal, Karnataka, 576104
P. Jagadish
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
Email: km.bhat@manipal.edu
Индия, Madhav Nagar, Manipal, Karnataka, 576104
Krishnamurthy Bhat
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
Автор, ответственный за переписку.
Email: km.bhat@manipal.edu
Индия, Madhav Nagar, Manipal, Karnataka, 576104