Analytical Method Development and Validation for HPLC-ECD Determination of Moxifloxacin in Marketed Formulations
- Authors: Phani Sekhar Reddy G.1, Navyasree K.S.1, Jagadish P.C.1, Bhat K.1
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Affiliations:
- Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
- Issue: Vol 52, No 7 (2018)
- Pages: 674-679
- Section: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/245400
- DOI: https://doi.org/10.1007/s11094-018-1879-1
- ID: 245400
Cite item
Abstract
An HPLC-ECD analytical method with high reproducibility and wide linearity range has been developed and validated. Moxifloxacin was separated and identified using this method with a simple mobile phase comprising Britton Robinson buffer pH 5.0 and methanol (93: 7 v/v) flowing at a rate of 0.5 mL/min through Acclaim C18 column (150 mm × 4.6 mm × 5 im) maintained at 35°C and detected at redox potential value of 1.0 V. The LOD and LOQ were found to be 2.2 and 6.6 μg/mL, respectively. The HPLC-ECD method of moxifloxacin analysis was validated as per ICH Q2R1 guidelines and applied for assay of marketed moxifloxacin formulations to establish the acceptable recovery.
About the authors
G. Phani Sekhar Reddy
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
Email: km.bhat@manipal.edu
India, Madhav Nagar, Manipal, Karnataka, 576104
K. S. Navyasree
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
Email: km.bhat@manipal.edu
India, Madhav Nagar, Manipal, Karnataka, 576104
P. C. Jagadish
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
Email: km.bhat@manipal.edu
India, Madhav Nagar, Manipal, Karnataka, 576104
Krishnamurthy Bhat
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
Author for correspondence.
Email: km.bhat@manipal.edu
India, Madhav Nagar, Manipal, Karnataka, 576104