Alkaloid Content, Antioxidant and Cytotoxic Activities of Various Parts of Papaver somniferum
- Авторы: Sharopov F.1,2, Valiev A.1, Gulmurodov I.1, Sobeh M.2, Satyal P.3, Wink M.2
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Учреждения:
- Department of Pharmaceutical Technology, Avicenna Tajik State Medical University
- Institute of Pharmacy and Molecular Biotechnology, Heidelberg University
- Department of Chemistry, University of Alabama in Huntsville
- Выпуск: Том 52, № 5 (2018)
- Страницы: 459-463
- Раздел: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/245244
- DOI: https://doi.org/10.1007/s11094-018-1839-9
- ID: 245244
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Аннотация
Poppy (Papaver somniferum) is a traditional source of isoquinoline alkaloids, especially morphine, codeine and papaverine that exhibit a wide spectrum of therapeutic effects. Due to beneficial properties, poppy has been used by humankind for several thousand years and is still being used around the world. The present study was aimed to investigate alkaloid composition and biological activity of various parts of the plant (leaves, stems, roots, capsules, flowers, seeds) in an ornamental variety of P. somniferum. The alkaloid content rangedwithin 0.16 – 6.5 mg/g fresh weight of raw plant material. The major components of the alkaloid fraction were papaverine (37.7 – 2062.9 μg/g), codeine (7.4 – 1280.5 μg/g) and morphine (3.25 – 929.3 μg/g). The P. somniferum alkaloid extract produced a strong antioxidant effect, which was evaluated using the radical-scavenging DPPH, ABTS and FRAP assays. The antioxidant properties were characterized by IC50 values ranging within 35.1 – 157.6 μg/mL for DPPH radical and 138.5 – 306.3 μg/mL for ABTS∙•+ radical scavenging. The ferric reducing antioxidant power (FRAP) values varied from 59.75 to 1348.71 mM FeSO4/g extract. The alkaloid extract was active against all human tumor cell lines studied (HeLa, Caco-2, MCF-7, CCRF-CEM and CEM/ADR 5000). The methanol extract exhibited a pronounced cytotoxicity against most of cancer cell lines studied, especially those with a low expression of ABC transporters.
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Об авторах
Farukh Sharopov
Department of Pharmaceutical Technology, Avicenna Tajik State Medical University; Institute of Pharmacy and Molecular Biotechnology, Heidelberg University
Автор, ответственный за переписку.
Email: sharopov@uni-heidelberg.de
Таджикистан, Rudaki 139, Dushanbe, 734003; Im Neuenheimer Feld 364, Heidelberg, D-69120
Abdujabbor Valiev
Department of Pharmaceutical Technology, Avicenna Tajik State Medical University
Email: sharopov@uni-heidelberg.de
Таджикистан, Rudaki 139, Dushanbe, 734003
Isomiddin Gulmurodov
Department of Pharmaceutical Technology, Avicenna Tajik State Medical University
Email: sharopov@uni-heidelberg.de
Таджикистан, Rudaki 139, Dushanbe, 734003
Mansour Sobeh
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University
Email: sharopov@uni-heidelberg.de
Германия, Im Neuenheimer Feld 364, Heidelberg, D-69120
Prabodh Satyal
Department of Chemistry, University of Alabama in Huntsville
Email: sharopov@uni-heidelberg.de
США, Huntsville, AL, 35899
Michael Wink
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University
Email: sharopov@uni-heidelberg.de
Германия, Im Neuenheimer Feld 364, Heidelberg, D-69120