Extraction of Mangiferin from the Leaves of the Mango Tree Mangifera indica and Evaluation of its Biological Activity in Terms of Blockade of α-glucosidase


Цитировать

Полный текст

Открытый доступ Открытый доступ
Доступ закрыт Доступ предоставлен
Доступ закрыт Только для подписчиков

Аннотация

A method of extracting mangiferin from dried powdered leaves from mango trees using the solvent mixture isopropanol and water (60:40 v/v) was used. The yields of technical product and purified mangiferin recrystallized from a mixture of isopropanol and water (50:50 v/v) were 1.46% (purity 88.6%) and 0.8% (purity 98.2%). The chemical structure of mangiferin was confirmed by UV-VIS and IR spectrophotometry, electrospray mass spectrometry, and 1H and 13C NMR spectroscopy. Technical product and mangiferin were tested for biological activity in terms of blockade of α-glucosidase, as compared with acarbose. Mangiferin provided significantly greater blockade of α-glucosidase than acarbose, with IC50 values of 11.18 and 5.82 μg/ml for technical product and mangiferin respectively and 199.47 μg/ml for acarbose. These results are important preliminary investigations for further investigation of mangiferin and the development of therapeutic formulations of this highly active hypoglycemic substance.

Об авторах

T. Vo

Department of Biotechnology Russian-Vietnamese Tropical Center

Автор, ответственный за переписку.
Email: hoaithu@mail.ru
Вьетнам, Hanoi, 10000

T. Nguyen

Department of Biotechnology Russian-Vietnamese Tropical Center

Email: hoaithu@mail.ru
Вьетнам, Hanoi, 10000

Q. Nguyen

Department of Biology, Defense Institute for Chemistry and the Environment

Email: hoaithu@mail.ru
Вьетнам, Hanoi, 10000

N. Ushakova

A. N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences

Email: hoaithu@mail.ru
Россия, 33 Lenin Prospekt, Moscow, 119071


© Springer Science+Business Media, LLC, part of Springer Nature, 2017

Данный сайт использует cookie-файлы

Продолжая использовать наш сайт, вы даете согласие на обработку файлов cookie, которые обеспечивают правильную работу сайта.

О куки-файлах