Extraction of Mangiferin from the Leaves of the Mango Tree Mangifera indica and Evaluation of its Biological Activity in Terms of Blockade of α-glucosidase
- Autores: Vo T.1, Nguyen T.1, Nguyen Q.2, Ushakova N.3
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Afiliações:
- Department of Biotechnology Russian-Vietnamese Tropical Center
- Department of Biology, Defense Institute for Chemistry and the Environment
- A. N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences
- Edição: Volume 51, Nº 9 (2017)
- Páginas: 806-810
- Seção: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/244810
- DOI: https://doi.org/10.1007/s11094-017-1697-x
- ID: 244810
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Resumo
A method of extracting mangiferin from dried powdered leaves from mango trees using the solvent mixture isopropanol and water (60:40 v/v) was used. The yields of technical product and purified mangiferin recrystallized from a mixture of isopropanol and water (50:50 v/v) were 1.46% (purity 88.6%) and 0.8% (purity 98.2%). The chemical structure of mangiferin was confirmed by UV-VIS and IR spectrophotometry, electrospray mass spectrometry, and 1H and 13C NMR spectroscopy. Technical product and mangiferin were tested for biological activity in terms of blockade of α-glucosidase, as compared with acarbose. Mangiferin provided significantly greater blockade of α-glucosidase than acarbose, with IC50 values of 11.18 and 5.82 μg/ml for technical product and mangiferin respectively and 199.47 μg/ml for acarbose. These results are important preliminary investigations for further investigation of mangiferin and the development of therapeutic formulations of this highly active hypoglycemic substance.
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Sobre autores
T. Vo
Department of Biotechnology Russian-Vietnamese Tropical Center
Autor responsável pela correspondência
Email: hoaithu@mail.ru
Vietnã, Hanoi, 10000
T. Nguyen
Department of Biotechnology Russian-Vietnamese Tropical Center
Email: hoaithu@mail.ru
Vietnã, Hanoi, 10000
Q. Nguyen
Department of Biology, Defense Institute for Chemistry and the Environment
Email: hoaithu@mail.ru
Vietnã, Hanoi, 10000
N. Ushakova
A. N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences
Email: hoaithu@mail.ru
Rússia, 33 Lenin Prospekt, Moscow, 119071