Assessment of 5-substituted Isatin as Surface Recognition Group: Design, Synthesis, and Antiproliferative Evaluation of Hydroxamates as Novel Histone Deacetylase Inhibitors
- Autores: Singh A.1, Raghuwanshi K.1, Patel V.1, Jain D.1, Veerasamy R.2, Dixit A.3, Rajak H.1
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Afiliações:
- Institute of Pharmaceutical Sciences, Guru Ghasidas University
- Faculty of Pharmacy, AIMST University
- Institute of Life Sciences
- Edição: Volume 51, Nº 5 (2017)
- Páginas: 366-374
- Seção: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/244612
- DOI: https://doi.org/10.1007/s11094-017-1616-1
- ID: 244612
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Resumo
Histone deacetylase (HDAC) is a promising target for cancer treatment. HDAC inhibitors consist of three pharmacophoric features: an aromatic cap group, zinc binding group (ZBG), and a linker chain connecting cap group to ZBG. Herein, we report on (i) substituted isatin moiety as the cap group that recognizes the surface of active enzyme pocket and (ii) thiosemicarbazide moiety incorporated as linker group responsible for connecting the cap group to ZBG (hydroxamic acid). The synthesized compounds were evaluated for their antiproliferative activity and HDAC enzyme inhibition. The binding mode analysis of proposed compounds was evaluated by docking studies. Several analogs were found to inhibit HDAC and cellular proliferation of Hela cervical cancer cells, with GI50 values in the micromolar range. One compound (Vd) was found to have greater in vitro antiproliferative activity in comparison to other compounds.
Sobre autores
Avineesh Singh
Institute of Pharmaceutical Sciences, Guru Ghasidas University
Email: harishdops@yahoo.co.in
Índia, Bilaspur (C. G.), 495009
Kamlesh Raghuwanshi
Institute of Pharmaceutical Sciences, Guru Ghasidas University
Email: harishdops@yahoo.co.in
Índia, Bilaspur (C. G.), 495009
Vijay Patel
Institute of Pharmaceutical Sciences, Guru Ghasidas University
Email: harishdops@yahoo.co.in
Índia, Bilaspur (C. G.), 495009
Deepak Jain
Institute of Pharmaceutical Sciences, Guru Ghasidas University
Email: harishdops@yahoo.co.in
Índia, Bilaspur (C. G.), 495009
Ravichandran Veerasamy
Faculty of Pharmacy, AIMST University
Email: harishdops@yahoo.co.in
Malásia, Semeling, 08100 Bedong, Darul Aman, Kedah
Anshuman Dixit
Institute of Life Sciences
Email: harishdops@yahoo.co.in
Índia, Bhubaneswar, Odisha, 751023
Harish Rajak
Institute of Pharmaceutical Sciences, Guru Ghasidas University
Autor responsável pela correspondência
Email: harishdops@yahoo.co.in
Índia, Bilaspur (C. G.), 495009