Ultrafiltration-Based Extraction and HPLC Analysis of Naproxen Sodium in Human Plasma Samples: an Innovative Approach to Pharmaceutical Analysis
- Авторлар: Çelebier M.1, Nenni M.1,2, Altınöz S.1
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Мекемелер:
- Hacettepe University, Faculty of Pharmacy, Department of Analytical Chemistry
- Çukurova University, Faculty of Pharmacy, Department of Analytical Chemistry
- Шығарылым: Том 50, № 4 (2016)
- Беттер: 275-279
- Бөлім: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/244362
- DOI: https://doi.org/10.1007/s11094-016-1437-7
- ID: 244362
Дәйексөз келтіру
Аннотация
The purpose of this study was to assess the possibility of using ultrafiltration-based extraction for pharmaceutical analysis and develop an innovative, rapid and simple analysis technique for determination of naproxen sodium in human plasma. The proposed extraction technique can be adapted for wide range of active pharmaceutical ingredients including water-soluble nonsteroidal anti-inflammatory drugs. Naproxen sodium was isolated from human plasma by ultrafiltration-based extraction and analyzed by HPLC. Proteins in the plasma samples were precipitated with methanol and the supernatant was filtered through commercial centrifugal filters (<3 kDa). The filtered part was vacuum centrifuged, dried, and then dissolved in water and centrifuged again. The supernatant was injected into the HPLC system where an ACE 5 μm C18 100 Å LC column (150 × 4.6 mm) was used. The mobile phase was acetonitrile – phosphate buffer (pH 3.0, 20 mM) 55 : 45 v/v mixture, the injection volume was 20 μL, and the UV detection was performed at 240 nm. Using the developed extraction technique combined with HPLC method, plasma samples containing naproxen sodium were successfully analyzed (RSD 1.04–6.47, bias 2.33–6.00).
Негізгі сөздер
Авторлар туралы
Mustafa Çelebier
Hacettepe University, Faculty of Pharmacy, Department of Analytical Chemistry
Хат алмасуға жауапты Автор.
Email: celebier@hacettepe.edu.tr
Түркия, Ankara
Merve Nenni
Hacettepe University, Faculty of Pharmacy, Department of Analytical Chemistry; Çukurova University, Faculty of Pharmacy, Department of Analytical Chemistry
Email: celebier@hacettepe.edu.tr
Түркия, Ankara; Adana
Sacide Altınöz
Hacettepe University, Faculty of Pharmacy, Department of Analytical Chemistry
Email: celebier@hacettepe.edu.tr
Түркия, Ankara