Development and Validation of a Quantitative Determination Method for the Antitubercular Drug PBTZ169 in Biological Media
- Authors: Stepanova E.S.1, Barsegyan S.S.1, Makarenkova L.M.1, Chistyakov V.V.1
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Affiliations:
- People’s Friendship University of Russia, Common Use Center
- Issue: Vol 52, No 3 (2018)
- Pages: 248-253
- Section: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/245106
- DOI: https://doi.org/10.1007/s11094-018-1801-x
- ID: 245106
Cite item
Abstract
An HPLC-MS/MS method for quantitative determination of the new antituberculosis drug PBTZ169 in blood serum, urine, and feces using perindopril as an internal standard was developed and validated. The method was selective, specific, accurate, and precise. Calibration curves were linear in the ranges 2 – 2,000, 5 – 3,000, and 100 – 10,000 ng/mL for blood serum, urine, and feces, respectively, with correlation coefficients >0.99. The detection limits of PBTZ169 were 1, 2.5, and 50 ng/mL for serum, urine, and feces, respectively. The limits of quantitation were 2 ng/mL in serum; 5, urine; and 100, feces.
Keywords
About the authors
E. S. Stepanova
People’s Friendship University of Russia, Common Use Center
Author for correspondence.
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 117198
S. S. Barsegyan
People’s Friendship University of Russia, Common Use Center
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 117198
L. M. Makarenkova
People’s Friendship University of Russia, Common Use Center
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 117198
V. V. Chistyakov
People’s Friendship University of Russia, Common Use Center
Email: stepanova25@inbox.ru
Russian Federation, Moscow, 117198