Validation of Spectrophotometric Method for Determination of Thiamazole in Liquids by Dissolution Test for the Transdermal Form
- Authors: Datkhayev U.M.1, Sakipova Z.B.1, Ustenova G.O.1, Zhakipbekov K.S.1, Kozhanova K.K.1, Kapsalyamova E.N.1, Datkayeva G.M.2, Ibadullayeva G.S.1, Sapakbay M.M.3, Bekenov K.E.4, Tulemissov S.K.1, Blatov R.M.1
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Affiliations:
- Asfendiyarov Kazakh National Medical University
- Ahmet Yesevi International Kazakh-Turkish University
- South – Kazakhstan State Pharmaceutical Academy
- West-Kazakhstan Marat Ospanov State Medical University
- Issue: Vol 53, No 6 (2019)
- Pages: 572-576
- Section: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/245873
- DOI: https://doi.org/10.1007/s11094-019-02039-3
- ID: 245873
Cite item
Abstract
The purpose of this work was to validate the method of UV absorption spectrophotometry for the determination of thiamazole in transdermal patches during their pharmaceutical development. According to the pharmacopoeial requirements of the Republic of Kazakhstan, dissolution test was used to assess the quality of drug product. Measuring the optical density of thiamazole solutions at a wavelength of 252 nm in the range of concentrations from 0.005 to 0.04 mg/mL showed that the optimal test solution concentration is 0.005 mg/mL. The proposed method was validated through the standard and drug recovery method for linearity, range, accuracy and precision. Optimized and validated, it demonstrated high specificity and accuracy for transdermal patch with thiamazole. The obtained results proved that the method could be used for routine analysis as well as for pharmaceutical development and quality control for thiamazole patches.
About the authors
Ubaidilla M. Datkhayev
Asfendiyarov Kazakh National Medical University
Email: zhakipbekovk@yahoo.com
Kazakhstan, Almaty
Zuriadda B. Sakipova
Asfendiyarov Kazakh National Medical University
Email: zhakipbekovk@yahoo.com
Kazakhstan, Almaty
Gulbaram O. Ustenova
Asfendiyarov Kazakh National Medical University
Email: zhakipbekovk@yahoo.com
Kazakhstan, Almaty
Kairat S. Zhakipbekov
Asfendiyarov Kazakh National Medical University
Author for correspondence.
Email: zhakipbekovk@yahoo.com
Kazakhstan, Almaty
Kaldanay K. Kozhanova
Asfendiyarov Kazakh National Medical University
Email: zhakipbekovk@yahoo.com
Kazakhstan, Almaty
Elmira N. Kapsalyamova
Asfendiyarov Kazakh National Medical University
Email: zhakipbekovk@yahoo.com
Kazakhstan, Almaty
Gulmira M. Datkayeva
Ahmet Yesevi International Kazakh-Turkish University
Email: zhakipbekovk@yahoo.com
Kazakhstan, Turkestan
Galiya S. Ibadullayeva
Asfendiyarov Kazakh National Medical University
Email: zhakipbekovk@yahoo.com
Kazakhstan, Almaty
Malik M. Sapakbay
South – Kazakhstan State Pharmaceutical Academy
Email: zhakipbekovk@yahoo.com
Kazakhstan, Shymkent
Kudaibergen E. Bekenov
West-Kazakhstan Marat Ospanov State Medical University
Email: zhakipbekovk@yahoo.com
Kazakhstan, Aktobe
Saken K. Tulemissov
Asfendiyarov Kazakh National Medical University
Email: zhakipbekovk@yahoo.com
Kazakhstan, Almaty
Ravil M. Blatov
Asfendiyarov Kazakh National Medical University
Email: zhakipbekovk@yahoo.com
Kazakhstan, Almaty