Angionorm Complex Herbal Preparation Constituents Identified by High Performance Liquid Chromatography-Mass Spectrometry

Abstract

Angionorm is an original Russian preparation based on the dried aqueous EtOH (25%) extract of a herbal mixture containing horse chestnuts (Aesculus hippocastanum L.), licorice roots (Glycyrrhiza glabra L.), hawthorn fruit (Crataegus), and dog-rose hips (Rosa canina). Areversed-phase HPLC-MS-MS procedure with gradient elution was developed to identify the main constituents in extracts of the mixture and each raw material type. Standards helped to identify 15 compounds of various classes (phenolic and hydroxycinnamic acids and their esters, flavonoids, flavonoid glycosides, triterpene saponins). Peaks of hyperoside, liquiritin, â-glycyrrhizic acid, and escin isomers were identified using MRM transitions, elution order, and literature data. Herbal sources of identified constituents were determined. Marker compounds characteristic of each raw-material type in the mixture extract were proposed.