Modified Poly(T-Butyl Methacrylate) as a Doxorubicin Carrier for Targeted Delivery
- Authors: Zhukova O.V.1, Sergeeva T.F.1, Gavrina A.I.1
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Affiliations:
- Privolzhsky Research Medical University, Ministry of Health of the Russian Federation
- Issue: Vol 52, No 6 (2018)
- Pages: 539-544
- Section: Drug Synthesis Methods and Manufacturing Technology
- URL: https://journals.rcsi.science/0091-150X/article/view/245318
- DOI: https://doi.org/10.1007/s11094-018-1856-8
- ID: 245318
Cite item
Abstract
Poly(t-butyl methacrylate) (PTBMA) was selected as a doxorubicin carrier for targeted delivery because it can hydrolyze to form water-soluble polymers. A chain-transfer reaction with thioglycolic acid was used. The thioglycolic acid concentration was 1 mass%, at which a polymer with the optimal biocompatibility (molecular mass ~12 kDa, polydispersion index Mw/Mn ≈ 1.5) was obtained. The polymer was chemically modified by a folate vector. The resulting polymer was converted via acid hydrolysis into a water-soluble copolymer. Then, a conjugate of doxorubicin (DOX) with the copolymer of t-butyl methacrylate and methacrylic acid modified by the folate vector on the terminal carboxylates was prepared. The degree of DOX conjugation to the polymer was 62.7 ± 11.8%. DOX was released from the polymer system almost three times faster at pH 4.6 than at pH 7.4. The cytotoxicity of the polymer conjugate was 1.52 μM.
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About the authors
O. V. Zhukova
Privolzhsky Research Medical University, Ministry of Health of the Russian Federation
Author for correspondence.
Email: ov-zhukova@mail.ru
Russian Federation, 10/1 Minina and Pozharskogo Sq, Nizhnii Novgorod, 603950
T. F. Sergeeva
Privolzhsky Research Medical University, Ministry of Health of the Russian Federation
Email: ov-zhukova@mail.ru
Russian Federation, 10/1 Minina and Pozharskogo Sq, Nizhnii Novgorod, 603950
A. I. Gavrina
Privolzhsky Research Medical University, Ministry of Health of the Russian Federation
Email: ov-zhukova@mail.ru
Russian Federation, 10/1 Minina and Pozharskogo Sq, Nizhnii Novgorod, 603950