Quantitative Determination of Total Saponins in Aralia Mandshurica Plant Raw Material

Abstract

Amethod for quantitative determination of total saponins in Aralia elata (Miq.) Seem. (A. mandshurica Rupr. et Maxim.) roots was developed using spectrophotometry at 510 nm of products from the reaction of the analytes with conc. H₂SO₄ as recalculated for saparal (total ammonium salts of aralia saponins) or the average specific absorption coefficient of saparal. The total saponin contents in the raw material varied from 9.41 ± 0.18 to 10.46 ± 0.15%. The error of a single determination using the developed method with confidence probability 95% was less than ± 1.43%.