Development and Validation of an HPLC-UV Method for Anilocaine Determination in Blood Plasma

Abstract

A method for anilocaine determination in rat blood plasma using HPLC with UV-detection was developed. Samples were prepared by liquid—liquid extraction with 1-BuOH—hexane (2:98, v/v) followed by stripping with aqueous formic acid (0.1%). Chromatographic analysis used a Nucleodur HILIC column eluted in isocratic mode by a mobile phase of MeCN and aqueous ammonium formate (25 mM) (85:15) and UV detection at 205 nm. The method was validated for selectivity, carry-over, calibration curve linearity, precision, accuracy, limit of quantitation, dilution integrity, and stability. The analytical range was 25 – 1,000 ng/mL. The proposed method could be applied to pharmacokinetic studies of anilocaine preparations.