Email this article Development of an HPLC-UV Method for Quantitative Determination of Acetylsalicylic Acid and Its Main Metabolite
- Authors: Belov V.Y.1, Kursakov S.V.1, Sevast’yanov V.I.1,2, Antonov E.N.3, Bogorodskii S.É.3, Popov V.K.3
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Affiliations:
- Institute of Biomedical Research and Technology
- V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
- Institute of Laser and Information Technologies, Russian Academy of Sciences
- Issue: Vol 52, No 2 (2018)
- Pages: 151-155
- Section: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/245038
- DOI: https://doi.org/10.1007/s11094-018-1781-x
- ID: 245038
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Abstract
An HPLC-UV method for determination of acetylsalicylic acid and its main metabolite, salicylic acid, in a model solution and in rabbit blood plasma was developed. Plasma samples were prepared by salting out. Chromatographic analysis was performed in isocratic mode over a Hypersil BDS C18 column using mobile phase MeCN—H2O (pH 2.5, 30:70) with detection at 230 nm. The limit of quantitation for acetylsalicylic and salicylic acids in the model solution was 0.05 μg/mL; in blood plasma, 0.2 μg/mL. The developed method was applied to the development of new acetylsalicylic-acid dosage forms based on biocompatible polymer carriers, including pharmacokinetic studies after i.m. implantation.
About the authors
V. Yu. Belov
Institute of Biomedical Research and Technology
Email: popov@laser.ru
Russian Federation, Moscow, 123557
S. V. Kursakov
Institute of Biomedical Research and Technology
Email: popov@laser.ru
Russian Federation, Moscow, 123557
V. I. Sevast’yanov
Institute of Biomedical Research and Technology; V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Email: popov@laser.ru
Russian Federation, Moscow, 123557; Moscow, 123182
E. N. Antonov
Institute of Laser and Information Technologies, Russian Academy of Sciences
Email: popov@laser.ru
Russian Federation, Shatura, Moscow Oblast, 140700
S. É. Bogorodskii
Institute of Laser and Information Technologies, Russian Academy of Sciences
Email: popov@laser.ru
Russian Federation, Shatura, Moscow Oblast, 140700
V. K. Popov
Institute of Laser and Information Technologies, Russian Academy of Sciences
Author for correspondence.
Email: popov@laser.ru
Russian Federation, Shatura, Moscow Oblast, 140700
