Isolation of Peptides from Calf Brain Tissue and Evaluation of their Tissue-Specific and Stimulatory Activity
- Authors: Chernova I.A.1, Zhilinskii D.V.2, Chalisova N.I.3, Shataeva L.K.1
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Affiliations:
- Institute of Macromolecular Compounds, Russian Academy of Sciences
- BIOCAD Biopharmaceutical Co.
- I. P. Pavlov Institute of Physiology, Russian Academy of Sciences
- Issue: Vol 51, No 6 (2017)
- Pages: 434-438
- Section: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/244642
- DOI: https://doi.org/10.1007/s11094-017-1628-x
- ID: 244642
Cite item
Abstract
A method for separating a mixture of calf brain peptides by extraction with aqueous HOAc followed by fractionation of the extract by cross-flow (tangential) microfiltration and solid-phase extraction was developed. The isolated peptide mixture contained constituents with molecular masses within 0.1 – 3 kDa. Low-pressure ion-exchange chromatography of this mixture over Dowex 50WX8 sulfonated cation exchanger isolated a pure peptide consisting of the five amino acids Arg, Lys, Gly, Ser, and Leu. The biological activity of the obtained pentapeptide was estimated on organotypic brain slice culture explants of Wistar rats. It was established that the peptide possessed high tissue-specific and stimulatory activity for brain explants that was greater than those of the commercial preparations Cortexin and Cerebrolysin.
About the authors
I. A. Chernova
Institute of Macromolecular Compounds, Russian Academy of Sciences
Author for correspondence.
Email: irina.chernova@gmail.com
Russian Federation, St. Petersburg, 199004
D. V. Zhilinskii
BIOCAD Biopharmaceutical Co.
Email: irina.chernova@gmail.com
Russian Federation, St. Petersburg, Strelna, 198515
N. I. Chalisova
I. P. Pavlov Institute of Physiology, Russian Academy of Sciences
Email: irina.chernova@gmail.com
Russian Federation, St. Petersburg, 199034
L. K. Shataeva
Institute of Macromolecular Compounds, Russian Academy of Sciences
Email: irina.chernova@gmail.com
Russian Federation, St. Petersburg, 199004