Synthesis and Pharmacological Properties of Linear Poly(Ethylene Glycol)s Conjugated to Interferon α-2b by Azo Coupling
- Authors: Sheremet’ev S.V.1, Lonshakov D.V.1, Korovkin S.A.1, Belosludtseva E.M.1, Katlinskii A.V.1, Semchenko A.V.1, Borisova O.V.2
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Affiliations:
- FORT LLC
- I. I. Mechnikov Research Institute of Vaccines and Sera, Russian Academy of Medical Sciences
- Issue: Vol 50, No 12 (2017)
- Pages: 788-793
- Section: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/244479
- DOI: https://doi.org/10.1007/s11094-017-1532-4
- ID: 244479
Cite item
Abstract
A method for the preparation of pharmacologically active conjugates of interferon α-2b with poly(ethylene glycol)s that absorbed in the visible spectral range was proposed. New covalent conjugates of linear poly(ethylene glycol)s with interferon α-2b were prepared via diazotization of 4-aminobenzoic acid poly(ethylene glycol) esters followed by azo coupling of the obtained diazonium salts with histidine imidazoles and tyrosine phenols of the protein amino-acid chain. The synthesized conjugates retained a high degree of interferon α-2b antiviral activity in vitro and had longer blood circulation times than a foreign analog and very low acute toxicity and immunogenicity.
Keywords
About the authors
S. V. Sheremet’ev
FORT LLC
Email: chem@folium.ru
Russian Federation, Ryazan, 390000
D. V. Lonshakov
FORT LLC
Email: chem@folium.ru
Russian Federation, Ryazan, 390000
S. A. Korovkin
FORT LLC
Email: chem@folium.ru
Russian Federation, Ryazan, 390000
E. M. Belosludtseva
FORT LLC
Email: chem@folium.ru
Russian Federation, Ryazan, 390000
A. V. Katlinskii
FORT LLC
Email: chem@folium.ru
Russian Federation, Ryazan, 390000
A. V. Semchenko
FORT LLC
Email: chem@folium.ru
Russian Federation, Ryazan, 390000
O. V. Borisova
I. I. Mechnikov Research Institute of Vaccines and Sera, Russian Academy of Medical Sciences
Email: chem@folium.ru
Russian Federation, Moscow, 105064