Use of Impedance Biotesting to Assess the Actions of Pharmaceutical Compounds on the Growth of Microorganisms

Abstract

A method for the analysis of microbial “growth curves” by real-time recording of changes in the impedance electrical conductance of the medium from multiple samples in parallel is described; these changes arise from metabolic processes taking place in the test microorganisms in the samples. Results obtained from practical application of the impedance biotesting method for analysis of the antibacterial activity of the aseptic agent chlorhexidine bigluconate are presented, along with results obtained using solutions of a catholyte and an anolyte obtained by processing 1% NaCl and Na₂SO₄ solutions in a specially constructed 2-liter eletrolyzer for 25 min with a current of I = 7 A and a voltage of V = 28 V.