Cytotoxic Activity of Atmospheric Cold Plasma Jet Towards 3D Human Breast Cancer Cell Model
- 作者: Patrakova E.1,2, Birykov M.1,2,3, Troitskaya O.1,3, Novak D.1,2,3, Milakhina E.4,3, Gugin P.5,3, Zakrevskyc D.4,5,3, Schweigert I.3, Koval O.1,2,3
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隶属关系:
- Institute of Chemical Biology and Fundamental Medicine, SB Russian Academy of Sciences
- Novosibirsk State University
- Khristianovich Institute of Theoretical and Applied Mechanics, SB Russian Academy of Sciences
- Novosibirsk State Technical University
- Rzhanov Institute of Semiconductor Physics, SB Russian Academy of Sciences
- 期: 卷 65, 编号 1 (2023)
- 页面: 39-53
- 栏目: Articles
- URL: https://journals.rcsi.science/0041-3771/article/view/140066
- DOI: https://doi.org/10.31857/S004137712301008X
- EDN: https://elibrary.ru/GLBBFZ
- ID: 140066
如何引用文章
详细
The treatment of solid tumors with a cold atmospheric plasma jet (CAP) is an innovative approach, which began to be actively developed only in the last decade. As a consequence, the studies aimed at revealing the conditions of selectivity of such effects on tumor cells, including in 3D tumor models, are important. It is known that the main cytotoxic effects of CAP are caused by reactive oxygen and nitrogen species, which are formed in the plasma flow and the availability of which for the cells in the classical 2D and 3D cultivation models may be different. We used multicellular spheroids of MCF7-EGFR cells with hyperexpression of epidermal growth factor receptor (EGFR), the parental MCF7 breast adenocarcinoma cell line, and MCF10A non-transformed human breast cells. Irradiation of MCF7-EGFR spheroids led to destruction of multicellular 3D structures into individual cells with activation of death processes. It was shown that cells of CAP-irradiated spheroids underwent phagocytosis by activated macrophages. When comparing direct exposure to CAP and cultivation of MCF7-EGFR spheroids in CAP-irradiated medium (CAP-IM), a higher content of reactive oxygen and nitrogen species in spheroid cells was found when cultured in CAP-IM, which further leads to a greater cytotoxic effect than in direct irradiation. The cytotoxicity of CAP-IM has been shown to be valid longer when such medium is stored at 4 than at −20°С. Thus, it was shown that the treatment of spheroids with CAP-IM was more effective in death induction than direct CAP irradiation.
作者简介
E. Patrakova
Institute of Chemical Biology and Fundamental Medicine, SB Russian Academy of Sciences; Novosibirsk State University
Email: biryukov.mm@ya.ru
Russia, 630090, Novosibirsk; Russia, 630090, Novosibirsk
M. Birykov
Institute of Chemical Biology and Fundamental Medicine, SB Russian Academy of Sciences; Novosibirsk State University; Khristianovich Institute of Theoretical and Applied Mechanics, SB Russian Academy of Sciences
编辑信件的主要联系方式.
Email: biryukov.mm@ya.ru
Russia, 630090, Novosibirsk; Russia, 630090, Novosibirsk; Russia, 630090, Novosibirsk
O. Troitskaya
Institute of Chemical Biology and Fundamental Medicine, SB Russian Academy of Sciences; Khristianovich Institute of Theoretical and Applied Mechanics, SB Russian Academy of Sciences
Email: biryukov.mm@ya.ru
Russia, 630090, Novosibirsk; Russia, 630090, Novosibirsk
D. Novak
Institute of Chemical Biology and Fundamental Medicine, SB Russian Academy of Sciences; Novosibirsk State University; Khristianovich Institute of Theoretical and Applied Mechanics, SB Russian Academy of Sciences
Email: biryukov.mm@ya.ru
Russia, 630090, Novosibirsk; Russia, 630090, Novosibirsk; Russia, 630090, Novosibirsk
E. Milakhina
Novosibirsk State Technical University; Khristianovich Institute of Theoretical and Applied Mechanics, SB Russian Academy of Sciences
Email: biryukov.mm@ya.ru
Russia, 630090, Novosibirsk; Russia, 630090, Novosibirsk
P. Gugin
Rzhanov Institute of Semiconductor Physics, SB Russian Academy of Sciences; Khristianovich Institute of Theoretical and Applied Mechanics, SB Russian Academy of Sciences
Email: biryukov.mm@ya.ru
Russia, 630090, Novosibirsk; Russia, 630090, Novosibirsk
D. Zakrevskyc
Novosibirsk State Technical University; Rzhanov Institute of Semiconductor Physics, SB Russian Academy of Sciences; Khristianovich Institute of Theoretical and Applied Mechanics, SB Russian Academy of Sciences
Email: biryukov.mm@ya.ru
Russia, 630090, Novosibirsk; Russia, 630090, Novosibirsk; Russia, 630090, Novosibirsk
I. Schweigert
Khristianovich Institute of Theoretical and Applied Mechanics, SB Russian Academy of Sciences
Email: biryukov.mm@ya.ru
Russia, 630090, Novosibirsk
O. Koval
Institute of Chemical Biology and Fundamental Medicine, SB Russian Academy of Sciences; Novosibirsk State University; Khristianovich Institute of Theoretical and Applied Mechanics, SB Russian Academy of Sciences
Email: biryukov.mm@ya.ru
Russia, 630090, Novosibirsk; Russia, 630090, Novosibirsk; Russia, 630090, Novosibirsk
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