Vimentin in Kolmer cells of spontaneously hypertensive rats

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Resumo

Epiplexus (Kolmer) cells are macrophage-like cells of the choroid plexus that help maintain the blood-cerebrospinal fluid barrier. Here we studied the structural organization of Kolmer cells in Wistar, Wistar-Kyoto and spontaneously hypertensive (SHR) rats. A comparative study using Iba-1, CD68 and vimentin immunohistochemistry showed that the functional activity of epiplexus cells differs in three examined groups of animals. Wistar-Kyoto and SHR rats showed noticeable signs of Kolmer cells activation, which consisted in the disappearance of cell processes resulting in the formation of round-shaped cells. Another significant observation was the presence of vimentin in activated epiplexus cells. The result obtained indicates that vimentin expression by phagocytic cells could be linked with their activation.

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Sobre autores

D. Korzhevskii

Institute of Experimental Medicine

Email: valeriya.raz@yandex.ru
Rússia, St. Petersburg

V. Razenkova

Institute of Experimental Medicine

Autor responsável pela correspondência
Email: valeriya.raz@yandex.ru
Rússia, St. Petersburg

O. Kirik

Institute of Experimental Medicine

Email: valeriya.raz@yandex.ru
Rússia, St. Petersburg

Bibliografia

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2. Fig. 1. Fragments of the vascular plexus of the lateral ventricles of the brain of Wistar (a), Wistar-Kyoto (b), and SHR (c) rats. Immunocytochemical reaction for Iba-1 protein with hematoxylin staining of nuclei. Plan-Apochromat 10×/0.22 objective. Arrows indicate Colmer cells. The scale bar is 20 μm

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3. Fig. 2. Vimentin-immunopositive cells associated with vascular plexus in the ventricles of Wistar (a), Wistar-Kyoto (b), SHR (c) rats. Immunocytochemical reaction for vimentin protein with hematoxylin staining of nuclei. Plan-Apochromat 100×/1.25 Oil objective (oil immersion). Arrows indicate vimentin-positive cells. The scale bar is 20 μm

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4. Fig. 3. Co-localization of Iba-1 and CD68 in cells of rat SHR vascular plexus on consecutive slices. Immunocytochemical reaction for Iba-1 (a) and CD68 (b) protein with hematoxylin staining of nuclei. Arrows indicate Colmer cells, asterisks are topographic markers indicating the same structures in a, b. Plan-Apochromat 40×/0.65 objective. The scale bar is 50 μm

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5. Fig. 4. Colmer cells in the vascular plexus of the lateral ventricle in the brain of Wistar (a, d), WKY (b, e) and SHR (c, f) rat lines. Double immunofluorescence staining for Iba-1 and vimentin, visualized with RRX (red) and Cy2 (green) fluorochromes, respectively. Confocal laser microscopy. Plan-Apochromat 63×/1.40 Oil DICM27 objective (oil immersion); a-c, general view. Frames delimit the areas shown in g-e. Scale bar is 10 μm. d, e, f - individual Colmer cells on the surface of the vascular plexus of rat lines Wistar (d), WKY (e), and SHR (f). The mass scale segment is 5 μm

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