Functional activity of blood neutrophilic granulocytes in patients with opisthorchiasis, depending on the severity of liver fibrosis
- 作者: Tsukanov V.1, Gorchilova E.2, Kolenchukova O.1, Gvozdev I.1, Savchenko A.1, Kasparov E.1, Vasyutin A.1, Tonkikh J.1, Borisov A.1, Rzhavicheva O.2
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隶属关系:
- Federal Research Center “Krasnoyarsk Scientific Center”
- Clinical hospital “RZD-Medicine” of the city of Krasnoyarsk
- 期: 卷 93, 编号 11 (2021)
- 页面: 1271-1277
- 栏目: Original articles
- URL: https://journals.rcsi.science/0040-3660/article/view/98419
- DOI: https://doi.org/10.26442/00403660.2021.11.201165
- ID: 98419
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Aim. To study the activity of neutrophilic granulocytes in patients with opisthorchiasis, depending on the severity of liver fibrosis.
Materials and methods. A total of 74 patients with chronic opisthorchiasis (39 men and 35 women, average age 42.3 years) and 32 practically healthy patients (17 men and 15 women, average age 41.5 years) aged 24 to 60 years were examined. Diagnosis of opisthorchiasis was carried out by two methods: coprooscopy and identification of eggs or bodies of adult parasites in duodenal content. Liver fibrosis was determined by the method of elastometry according to the METAVIR scale in all 74 patients with opisthorchiasis. The study of the functional activity of neutrophils in the blood was performed to all 74 patients with opisthorchiasis and 32 healthy individuals from the control group by chemiluminescent analysis with measurement of the reactive oxygen species (ROS) production intensity in a spontaneous and zymosan-induced reaction in lucigenin and luminol-dependent processes.
Results. Liver fibrosis F2 by METAVIR was registered in 20.3% of the examined individuals, liver fibrosis F3–F4 by METAVIR was detected in 17.6% of patients with opisthorchiasis. In patients with opisthorchiasis with liver fibrosis F3–F4 by METAVIR, a significant decrease in the functional activity of neutrophilic granulocytes was registered in comparison with individuals with liver fibrosis F0–F1 by METAVIR, as evidenced by a significant decrease in the maximum intensity of ROS production (Imax) and the area under the curve (S) chemiluminescence in lucigenin and luminol-dependent processes both in the spontaneous and zymosan-induced reaction.
Conclusion. These results provide new information to explain the mechanisms of liver fibrosis in patients opisthorchiasis and create opportunities for the development of diagnostics and preventive technologies.
作者简介
Vladislav Tsukanov
Federal Research Center “Krasnoyarsk Scientific Center”
编辑信件的主要联系方式.
Email: gastro@impn.ru
ORCID iD: 0000-0002-9980-2294
д-р мед. наук, проф., зав. клиническим отд-нием патологии пищеварительной системы взрослых и детей НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
俄罗斯联邦, Krasnoyarsk
Ekaterina Gorchilova
Clinical hospital “RZD-Medicine” of the city of Krasnoyarsk
Email: gastro@impn.ru
врач-гастроэнтеролог гастроэнтерологического отд-ния
俄罗斯联邦, Krasnoyarsk
Oksana Kolenchukova
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0001-9552-447X
д-р биол. наук, вед. науч. сотр. лаб. молекулярно-клеточной физиологии и патологии НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
俄罗斯联邦, Krasnoyarsk
Ivan Gvozdev
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0002-1041-9871
мл. науч. сотр. лаб. молекулярно- клеточной физиологии и патологии НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
俄罗斯联邦, Krasnoyarsk
Andrei Savchenko
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0001-5829-672X
д-р мед. наук, проф., зав. лаб. молекулярно-клеточной физиологии и патологии НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
俄罗斯联邦, Krasnoyarsk
Eduard Kasparov
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0002-5988-1688
д-р мед. наук, проф., дир. НИИ медицинских проблем Севера, зам. дир. по научно-организационной работе ФГБНУ ФИЦ КНЦ
俄罗斯联邦, Krasnoyarsk
Aleksandr Vasyutin
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0002-6481-3196
канд. мед. наук, ст. науч. сотр. клинического отд-ния патологии пищеварительной системы взрослых и детей НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
俄罗斯联邦, Krasnoyarsk
Juliia Tonkikh
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0001-7518-1895
канд. мед. наук, вед. науч. сотр. клинического отд-ния патологии пищеварительной системы взрослых и детей НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
俄罗斯联邦, KrasnoyarskAleksandr Borisov
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0002-9026-2615
канд. мед. наук, вед. науч. сотр. лаб. молекулярно-клеточной физиологии и патологии НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
俄罗斯联邦, Krasnoyarsk
Olga Rzhavicheva
Clinical hospital “RZD-Medicine” of the city of Krasnoyarsk
Email: gastro@impn.ru
канд. мед. наук, зав. гастроэнтерологическим отд-нием ЧУЗ «КБ “РЖД-Медицина” города Красноярск»
俄罗斯联邦, Krasnoyarsk
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