Email this article Functional activity of blood neutrophilic granulocytes in patients with opisthorchiasis, depending on the severity of liver fibrosis
- Authors: Tsukanov V.V.1, Gorchilova E.G.2, Kolenchukova O.A.1, Gvozdev I.I.1, Savchenko A.A.1, Kasparov E.V.1, Vasyutin A.V.1, Tonkikh J.L.1, Borisov A.G.1, Rzhavicheva O.S.2
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Affiliations:
- Federal Research Center “Krasnoyarsk Scientific Center”
- Clinical hospital “RZD-Medicine” of the city of Krasnoyarsk
- Issue: Vol 93, No 11 (2021)
- Pages: 1271-1277
- Section: Original articles
- URL: https://journals.rcsi.science/0040-3660/article/view/98419
- DOI: https://doi.org/10.26442/00403660.2021.11.201165
- ID: 98419
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Abstract
Aim. To study the activity of neutrophilic granulocytes in patients with opisthorchiasis, depending on the severity of liver fibrosis.
Materials and methods. A total of 74 patients with chronic opisthorchiasis (39 men and 35 women, average age 42.3 years) and 32 practically healthy patients (17 men and 15 women, average age 41.5 years) aged 24 to 60 years were examined. Diagnosis of opisthorchiasis was carried out by two methods: coprooscopy and identification of eggs or bodies of adult parasites in duodenal content. Liver fibrosis was determined by the method of elastometry according to the METAVIR scale in all 74 patients with opisthorchiasis. The study of the functional activity of neutrophils in the blood was performed to all 74 patients with opisthorchiasis and 32 healthy individuals from the control group by chemiluminescent analysis with measurement of the reactive oxygen species (ROS) production intensity in a spontaneous and zymosan-induced reaction in lucigenin and luminol-dependent processes.
Results. Liver fibrosis F2 by METAVIR was registered in 20.3% of the examined individuals, liver fibrosis F3–F4 by METAVIR was detected in 17.6% of patients with opisthorchiasis. In patients with opisthorchiasis with liver fibrosis F3–F4 by METAVIR, a significant decrease in the functional activity of neutrophilic granulocytes was registered in comparison with individuals with liver fibrosis F0–F1 by METAVIR, as evidenced by a significant decrease in the maximum intensity of ROS production (Imax) and the area under the curve (S) chemiluminescence in lucigenin and luminol-dependent processes both in the spontaneous and zymosan-induced reaction.
Conclusion. These results provide new information to explain the mechanisms of liver fibrosis in patients opisthorchiasis and create opportunities for the development of diagnostics and preventive technologies.
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##article.viewOnOriginalSite##About the authors
Vladislav V. Tsukanov
Federal Research Center “Krasnoyarsk Scientific Center”
Author for correspondence.
Email: gastro@impn.ru
ORCID iD: 0000-0002-9980-2294
д-р мед. наук, проф., зав. клиническим отд-нием патологии пищеварительной системы взрослых и детей НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
Russian Federation, Krasnoyarsk
Ekaterina G. Gorchilova
Clinical hospital “RZD-Medicine” of the city of Krasnoyarsk
Email: gastro@impn.ru
врач-гастроэнтеролог гастроэнтерологического отд-ния
Russian Federation, Krasnoyarsk
Oksana A. Kolenchukova
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0001-9552-447X
д-р биол. наук, вед. науч. сотр. лаб. молекулярно-клеточной физиологии и патологии НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
Russian Federation, Krasnoyarsk
Ivan I. Gvozdev
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0002-1041-9871
мл. науч. сотр. лаб. молекулярно- клеточной физиологии и патологии НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
Russian Federation, Krasnoyarsk
Andrei A. Savchenko
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0001-5829-672X
д-р мед. наук, проф., зав. лаб. молекулярно-клеточной физиологии и патологии НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
Russian Federation, Krasnoyarsk
Eduard V. Kasparov
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0002-5988-1688
д-р мед. наук, проф., дир. НИИ медицинских проблем Севера, зам. дир. по научно-организационной работе ФГБНУ ФИЦ КНЦ
Russian Federation, Krasnoyarsk
Aleksandr V. Vasyutin
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0002-6481-3196
канд. мед. наук, ст. науч. сотр. клинического отд-ния патологии пищеварительной системы взрослых и детей НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
Russian Federation, Krasnoyarsk
Juliia L. Tonkikh
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0001-7518-1895
канд. мед. наук, вед. науч. сотр. клинического отд-ния патологии пищеварительной системы взрослых и детей НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
Russian Federation, KrasnoyarskAleksandr G. Borisov
Federal Research Center “Krasnoyarsk Scientific Center”
Email: gastro@impn.ru
ORCID iD: 0000-0002-9026-2615
канд. мед. наук, вед. науч. сотр. лаб. молекулярно-клеточной физиологии и патологии НИИ медицинских проблем Севера ФГБНУ ФИЦ КНЦ
Russian Federation, Krasnoyarsk
Olga S. Rzhavicheva
Clinical hospital “RZD-Medicine” of the city of Krasnoyarsk
Email: gastro@impn.ru
канд. мед. наук, зав. гастроэнтерологическим отд-нием ЧУЗ «КБ “РЖД-Медицина” города Красноярск»
Russian Federation, Krasnoyarsk
References
- Puche JE, Saiman Y, Friedman SL. Hepatic stellate cells and liver fibrosis. Compr Physiol. 2013;3(4):1473-92. doi: 10.1002/cphy.c120035
- Elpek GÖ. Cellular and molecular mechanisms in the pathogenesis of liver fibrosis: an update. World J Gastroenterol. 2014;20(23):7260-76. doi: 10.3748/wjg.v20.i23.7260
- Xu R, Huang H, Zhang Z, Wang FS. The role of neutrophils in the development of liver diseases. Cell Mol Immunol. 2014;11(3):224-31. doi: 10.1038/cmi.2014.2
- Pillay J, Kamp VM, van Hoffen E, et al. A subset of neutrophils in human systemic inflammation inhibits T cell responses through Mac-1. J Clin Invest. 2012;122(1):327-36. doi: 10.1172/JCI57990
- Prueksapanich P, Piyachaturawat P, Aumpansub P, et al. Liver fluke-associated biliary tract cancer. Gut Liver. 2018;12(3):236-45. doi: 10.5009/gnl17102
- Цуканов В.В., Тонких Ю.Л., Гилюк А.В., и др. Диагностика, клиника и лечение описторхоза. Доктор.Ру. 2019;(8):49-53 [Tsukanov VV, Tonkikh YL, Gilyuk AV, et al. Diagnostika, klinika i lechenie opistorkhoza. Doctor.Ru. 2019;(8):49-53 (in Russian)]. doi: 10.31550/1727-2378-2019-163-8-49-53
- Poynard T, Bedossa P, Opolon P. Natural history of liver fibrosis progression in patients with chronic hepatitis C. The OBSVIRC, METAVIR, CLINIVIR, and DOSVIRC groups. Lancet. 1997;349(9055):825-32. doi: 10.1016/s0140-6736(96)07642-8
- Bouvard V, Baan R, Straif K, et al. A review of human carcinogens – Part B: biological agents. Lancet Oncol. 2009;10(4):321-2. doi: 10.1016/s1470-2045(09)70096-8
- Цуканов В.В., Горчилова Е.Г., Васютин А.В., и др. Современные принципы ведения больных с описторхозом. Врач. 2019;30(12):25-8 [Tsukanov VV, Gorchilova EG, Vasyutin AV, et al. Sovremennye printsipy vedeniia bol'nykh s opistorkhozom. Vrach. 2019;30(12):25-8 (in Russian)]. doi: 10.29296/25877305-2019-12-07
- Максимова Г.А., Жукова Н.А., Кашина Е.В., и др. Роль Opisthorchis felineus в индукции рака желчных протоков. Паразитология. 2015;49(1):3-11 [Maksimova GA, Zhukova NA, Kashina EV, et al. Rol' Opisthorchis felineus v induktsii raka zhelchnykh protokov. Parazitologiya. 2015;49(1):3-11 (in Russian)].
- Peng Y, Li Y, He Y, et al. The role of neutrophil to lymphocyte ratio for the assessment of liver fibrosis and cirrhosis: a systematic review. Expert Rev Gastroenterol Hepatol. 2018;12(5):503-13. doi: 10.1080/17474124.2018.1463158
- Rice J, Dodge JL, Bambha KM, et al. Neutrophil-to-lymphocyte ratio associates independently with mortality in hospitalized patients with cirrhosis. Clin Gastroenterol Hepatol. 2018;16(11):1786-91. doi: 10.1016/j.cgh.2018.04.045
- Bakshi SS. Neutrophil-to-lymphocyte ratio in patients with cirrhosis. Clin Gastroenterol Hepatol. 2019;17(4):799. doi: 10.1016/j.cgh.2018.09.009
- Jaeger BN, Donadieu J, Cognet C, et al. Neutrophil depletion impairs natural killer cell maturation, function, and homeostasis. J Exp Med. 2012;209(3):565-80. doi: 10.1084/jem.20111908
- Radaeva S, Sun R, Jaruga B, et al. Natural killer cells ameliorate liver fibrosis by killing activated stellate cells in NKG2D-dependent and tumor necrosis factor-related apoptosis-inducing ligand-dependent manners. Gastroenterology. 2006;130(2):435-52. doi: 10.1053/j.gastro.2005.10.055
- Melhem A, Muhanna N, Bishara A, et al. Anti-fibrotic activity of NK cells in experimental liver injury through killing of activated HSC. J Hepatol. 2006;45(1):60-71. doi: 10.1016/j.jhep.2005.12.025
- Tritto G, Bechlis Z, Stadlbauer V, et al. Evidence of neutrophil functional defect despite inflammation in stable cirrhosis. J Hepatol. 2011;55(3):574-81. doi: 10.1016/j.jhep.2010.11.034
