Leptin and leptin receptor evaluation in atherosclerotic plaques in patients with type 2 diabetes mellitus
- Authors: Dimitrova D.A.1, Mikhailov I.A.2, Tokarev K.Y.1, Michurova M.S.1, Gorbacheva A.M.1, Danilova N.V.2, Malkov P.G.2, Kalashnikov V.Y.1
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Affiliations:
- Endocrinology Research Centre
- Lomonosov Moscow State University
- Issue: Vol 93, No 10 (2021)
- Pages: 1186-1192
- Section: Original articles
- URL: https://journals.rcsi.science/0040-3660/article/view/86945
- DOI: https://doi.org/10.26442/00403660.2021.10.201079
- ID: 86945
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Abstract
Background. Diabetes mellitus (DM) is a significant predictor of atherosclerosis, cardiovascular disease, and cardiovascular mortality. It is known that atherosclerosis occurs earlier in patients with diabetes, reducing the duration of their life. Leptin as well as other inflammatory markers can contribute to the progression of atherosclerosis in patients with DM, participate in the development of a local inflammatory reaction.
Aim. Determine the cells immunophenotype of atherosclerotic plaques in patients with diabetes.
Materials and methods. We analyzed 24 patients (20 men and 4 women), who underwent aortofemoral bypass, femoral-tibial bypass or carotid endarterectomy. During the operation, a fragment of the arterial wall with an atherosclerotic plaque was obtained for further immunohistochemical studies. Five histologic plaque characteristics (CD68+, α-SMA, CD34, leptin and leptin receptor) were compared.
Results. No difference in the expression of CD68 (p=0.922), α-SMA (p=0.192), CD34 (p=0.858), leptin receptor (p=0.741) and leptin (p=0.610) in atherosclerotic plaques were observed between patients with and without DM. The lack of significant differences between the two groups was possibly due to the small number of observations with DM. In particular, when assessing the expression of selected markers in atherosclerotic plaques, patients with DM showed significantly more leptin receptors than patients without DM (2160.716 and 1205.88 respectively); and also significantly less CD68+ (0.39 and 0.98 respectively) and α-SMA+ (6.5 and 13.5 respectively).
Conclusion. Based on the expression of CD68, α-SMA, CD34, leptin receptor and leptin, no significant differences were observed in atherosclerotic plaque between patients with and without DM. At the same time, despite the limitations of the study (a small number of patients, moderate severity of DM, elderly patients in the DM group), we found a tendency in the increased number of leptin receptors and a decreased number of α-SMA+, CD68+ in DM atherosclerotic plaques. Further study needed, taking into account the limitations of this work.
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##article.viewOnOriginalSite##About the authors
Diana A. Dimitrova
Endocrinology Research Centre
Author for correspondence.
Email: diadavtyan@gmail.com
ORCID iD: 0000-0002-1359-8297
ассистент методического аккредитационно-симуляционного центра
Russian Federation, MoscowIlya A. Mikhailov
Lomonosov Moscow State University
Email: diadavtyan@gmail.com
ORCID iD: 0000-0001-8020-369X
стажер-исследователь отд. клинической патологии Медицинского научно-образовательного центра
Russian Federation, MoscowKonstantin Yu. Tokarev
Endocrinology Research Centre
Email: diadavtyan@gmail.com
канд. мед. наук, зав. операционным блоком отд-ния кардиологии, эндоваскулярной и сосудистой хирургии
Russian Federation, MoscowMarina S. Michurova
Endocrinology Research Centre
Email: diadavtyan@gmail.com
ORCID iD: 0000-0003-1495-5847
науч. сотр. врач-кардиолог отд. кардиологии и сосудистой хирургии
Russian Federation, MoscowAnna M. Gorbacheva
Endocrinology Research Centre
Email: diadavtyan@gmail.com
ORCID iD: 0000-0001-6581-4521
науч. сотр., врач-эндокринолог отд-ния патологии околощитовидных желез
Russian Federation, MoscowNatalia V. Danilova
Lomonosov Moscow State University
Email: diadavtyan@gmail.com
ORCID iD: 0000-0001-7848-6707
канд. мед. наук, ст. науч. сотр. отд. клинической патологии Медицинского научно-образовательного центра
Russian Federation, MoscowPavel G. Malkov
Lomonosov Moscow State University
Email: diadavtyan@gmail.com
ORCID iD: 0000-0001-5074-3513
д-р мед. наук, зав. отд. клинической патологии Медицинского научно-образовательного центра
Russian Federation, MoscowViktor Yu. Kalashnikov
Endocrinology Research Centre
Email: diadavtyan@gmail.com
ORCID iD: 0000-0001-5573-0754
чл.-кор. РАН, д-р мед. наук, проф., зам. дир. Центра по координации эндокринологической службы, зав. отд. кардиологии и сосудистой хирургии
Russian Federation, MoscowReferences
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