Multipotent mesenchymal stromal cells application for acute graft versus host disease treatment

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Abstract

Aim. Analysis of the effectiveness of the MSCs aministration as the second- or third-line therapy of acute GVHD (aGVHD) resistant to glucocorticosteroid treatment.

Materials and methods. The study included 35 patients who received MSCs obtained from the bone marrow of healthy donors as a treatment of steroid-resistant aGVHD. The clinical parameters of patients, MSCs’ cultural characteristics, the MSC expression profile for various genes including those involved in immunomodulation, expression of cells’ surface markers, the source of MSCs, as well as the frequency and number of MSC administrations were analyzed.

Results. Response to therapy was achieved in 74% of cases, a complete response was reached in 13 (37%) patients, partial response/clinical improvement was demonstrated in 13 (37%). This treatment was ineffective in 9 patients. The prediction of a group of patients with good response to MSC therapy turned to be impossible. The differences between the effective and ineffective for the GVHD treatment MSCs samples were found. The effective ones were characterized with a decreased total MSCs production and an increase in the main histocompatibility complex and PDL-1 antigens expression.

Conclusion. These data allow to select optimal samples for aGVHD treatment that can improve clinical results. aGVHD treatment with MSCs has shown efficacy comparable to other treatment approaches. Given the low percentage of complications and the absence of significant adverse effects, MSC therapy seems to be one of the optimal approaches to the treatment of resistant forms of GVHD.

About the authors

L. A. Kuzmina

National Research Center for Hematology

Author for correspondence.
Email: kuzlara@rambler.ru
ORCID iD: 0000-0001-6201-6276

к.м.н., зав. отд-нием интенсивной высокодозной химиотерапии и трансплантации костного мозга с круглосуточным и дневным стационарами

Russian Federation, Moscow

N. A. Petinati

National Research Center for Hematology

Email: kuzlara@rambler.ru
ORCID iD: 0000-0001-6591-3183

ст. науч. сотр. лаб. физиологии кроветворения

Russian Federation, Moscow

V. A. Vasilieva

National Research Center for Hematology

Email: kuzlara@rambler.ru
ORCID iD: 0000-0003-0904-7385

зав. отд-нием иммунохимиотерапии с дневным стационаром для больных после трансплантации костного мозга

Russian Federation, Moscow

M. V. Dovydenko

National Research Center for Hematology

Email: kuzlara@rambler.ru
ORCID iD: 0000-0002-6082-0110

врач-гематолог отд-ния интенсивной высокодозной химиотерапии и трансплантации костного мозга

Russian Federation, Moscow

M. Yu. Drokov

National Research Center for Hematology

Email: kuzlara@rambler.ru
ORCID iD: 0000-0001-9431-8316

рук. сектора по изучению иммунных воздействий и осложнений после трансплантации костного мозга

Russian Federation, Moscow

Yu. O. Davydova

National Research Center for Hematology

Email: kuzlara@rambler.ru
ORCID iD: 0000-0001-5932-0285

врач клинической лабораторной диагностики лаб. иммунофенотипирования клеток крови и костного мозга

Russian Federation, Moscow

N. M. Kapranov

National Research Center for Hematology

Email: kuzlara@rambler.ru
ORCID iD: 0000-0002-6512-910X

медицинский физик лаб. иммунофенотипирования клеток крови и костного мозга

Russian Federation, Moscow

N. V. Sats

National Research Center for Hematology

Email: kuzlara@rambler.ru
ORCID iD: 0000-0002-1559-9381

ст. науч. сотр. лаб. физиологии кроветворения

Russian Federation, Moscow

Yu. A. Chabaeva

National Research Center for Hematology

Email: kuzlara@rambler.ru
ORCID iD: 0000-0001-8044-598X

сотр. информационно-аналитического отд.

Russian Federation, Moscow

S. M. Kulikov

National Research Center for Hematology

Email: kuzlara@rambler.ru
ORCID iD: 0000-0002-6288-7570

зав. информационно-аналитическим отд.

Russian Federation, Moscow

T. V. Gaponova

National Research Center for Hematology

Email: kuzlara@rambler.ru
ORCID iD: 0000-0002-9684-5045

зам. ген. дир. по трансфузиологии, зав. отд. процессинга клеток крови и криоконсервирования

Russian Federation, Moscow

N. I. Drize

National Research Center for Hematology

Email: kuzlara@rambler.ru

зав. лаб. физиологии кроветворения

Russian Federation, Moscow

E. N. Parovichnikova

National Research Center for Hematology

Email: kuzlara@rambler.ru
ORCID iD: 0000-0001-6177-3566

зав. отд. химиотерапии гемобластозов, депрессий кроветворения и трансплантации костного мозга

Russian Federation, Moscow

V. G. Savchenko

National Research Center for Hematology

Email: kuzlara@rambler.ru
ORCID iD: 0000-0001-8188-5557

акад. РАН, д.м.н., проф., ген. дир.

Russian Federation, Moscow

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