Email this article Thermal heliox proteome. High-temperature heliox does not cause destruction of human respiratory system cells
- Authors: Varfolomeev S.D.1,2, Panin A.A.3, Ryabokon A.M.1,2, Kozyr A.S.2, Kononikhin A.S.2, Shogenova L.V.4,5, Chuchalin A.G.4
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Affiliations:
- Lomonosov Moscow State University
- Emanuel Institute of Biochemical Physics
- Medtechinnovations
- Pirogov Russian National Research Medical University
- Pletnev City Clinical Hospital
- Issue: Vol 92, No 6 (2020)
- Pages: 69-72
- Section: Original articles
- URL: https://journals.rcsi.science/0040-3660/article/view/34988
- DOI: https://doi.org/10.26442/00403660.2020.06.000769
- ID: 34988
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Abstract
Aim. Conducting a pilot study to assess the effect of thermal heliox on the state of the respiratory tract by studying of the exhaled breath condensate protein composition before the thermal heliox procedure, immediately after and after three hours of relaxation
Materials and methods. A comparative study of the exhaled breath condensates (EBC) protein composition of five non-smoking healthy donors was carried out. The EBC was taken before the respiratory procedure, immediately after a 20-minute inhalation by mixture of He/O2 gases (70/30) heated to 70°C and 3 hours later. The protein composition was determined by chromatography-mass spectrometric analysis after selective tryptic hydrolysis. The results were processed using the Mascot program and the UniProt database.
Results. After the heliox procedure, the volume of the collected condensate (1–1.5 ml) decreases by an average of 32% and is practically restored after three hours of relaxation. Most proteins were consistent for all samples, regardless of the thermal heliox procedure. These are keratins, several proteins of the immune system (immunoglobulins, compliment proteins), tubulin. In samples after thermal heliox, the appearance of small amounts of additional proteins is observed. These are proteins of muscle metabolism (actin and calmodulin), fibrinogen, traces of hemoglobin, apolipoprotein, type B creatine kinase. After three hours of relaxation, tubulin disappears in the EBC.
Conclusion. Most exhaled proteins are the same before, after the procedure, and for three hours of relaxation. The results obtained demonstrate the relative safety of the use of high temperature heliox as a therapeutic agent.
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##article.viewOnOriginalSite##About the authors
Sergey D. Varfolomeev
Lomonosov Moscow State University; Emanuel Institute of Biochemical Physics
Email: amryabokon@gmail.com
чл.-кор. РАН, д.х.н., проф., дир. Института физико-химических основ функционирования сети нейронов и искусственного интеллекта; науч. рук.
Russian Federation, MoscowAleksander A. Panin
Medtechinnovations
Email: amryabokon@gmail.com
д.э.н, ген. дир.
Russian Federation, MoscowAnna M. Ryabokon
Lomonosov Moscow State University; Emanuel Institute of Biochemical Physics
Author for correspondence.
Email: amryabokon@gmail.com
ORCID iD: 0000-0001-9043-9129
к.х.н., науч. сотр. каф. химической энзимологии химического фак-та; ст. науч. сотр.
Russian Federation, MoscowAnna S. Kozyr
Emanuel Institute of Biochemical Physics
Email: amryabokon@gmail.com
ORCID iD: 0000-0001-6723-2963
аспирант
Russian Federation, MoscowAleksey S. Kononikhin
Emanuel Institute of Biochemical Physics
Email: amryabokon@gmail.com
ORCID iD: 0000-0002-2238-3458
к.ф.-м.н., ст. науч. сотр
Russian Federation, MoscowLyudmila V. Shogenova
Pirogov Russian National Research Medical University; Pletnev City Clinical Hospital
Email: amryabokon@gmail.com
к.м.н., доц. каф. госпитальной терапии педиатрического фак-та; врач анестезиолог-реаниматолог отд-ния реанимации и интенсивной терапии
Aleksander G. Chuchalin
Pirogov Russian National Research Medical University
Email: amryabokon@gmail.com
ORCID iD: 0000-0002-5070-5450
Russian Federation, Moscow
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