Email this article DICER and DROSHA gene expression in peripheral mononuclear blood cells from rheumatoid arthritis patients under methotrexate therapy
- Authors: Malysheva IE1, Topchieva LV1, Balan OV1, Marusenko IM2,3, Barysheva OY2,3
-
Affiliations:
- Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, laboratory of genetics
- Federal State Budgetary Educational Institution of Higher Education «Petrozavodsk State University»
- Department of Hospital Therapy
- Issue: Vol 90, No 5 (2018)
- Pages: 72-75
- Section: Editorial
- URL: https://journals.rcsi.science/0040-3660/article/view/32761
- DOI: https://doi.org/10.26442/terarkh201890572-75
- ID: 32761
Cite item
Full Text
Abstract
The aim of this research was studying the level of DROSHA and DICER genes expression in peripheral mononuclear blood cells (PBMC) in rheumatoid arthritis (RA) patients under methotrexate therapy. Materials and methods. 82 people (from 45 to 70 years) enrolled in this study were divided into 3 groups (the first one - healthy donors (n=33 median age 49.93±1.87); the second group - rheumatoid arthritis patients without any therapy (n=15; median age 57.28±15.18) and the third group (n=34; median age 60.88±9.02) - rheumatoid arthritis patients who treated at least 4 weeks with methotrexate therapy (10-20 mg/week). The DICER and DROSHA genes expression level was determined by real-time PCR. Results. The number of DICER gene transcripts in PBMC in rheumatoid arthritis patients without therapy as in RA patients treated with methotrexate was reduced in comparison with the healthy donors (p<0.001 and p>0.05 respectively). The level of DROSHA gene expression in PBMC was not significantly different in all groups enrolled in this study (p>0.05). Conclusion. Our findings suggest that that the DICER gene expression level in perifheral mononuclear blood cells decreased with the development of rheumatoid arthritis. Methotrexate doesn’t influence on the mRNA level of this gene.
Full Text
##article.viewOnOriginalSite##About the authors
I E Malysheva
Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, laboratory of genetics
Email: i.e.malysheva@yandex.ru
с.н.с. лаб. генетики ИБ КарНЦ РАН Petrozavodsk, Russia
L V Topchieva
Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, laboratory of geneticsв.н.с. лаб. генетики ИБ КарНЦ РАН Petrozavodsk, Russia
O V Balan
Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, laboratory of geneticsс.н.с. лаб. генетики ИБ КарНЦ РАН Petrozavodsk, Russia
I M Marusenko
Federal State Budgetary Educational Institution of Higher Education «Petrozavodsk State University»; Department of Hospital Therapyд.м.н., проф., каф. госпитальной терапии Петрозаводского государственного университета Petrozavodsk, Russia
O Y Barysheva
Federal State Budgetary Educational Institution of Higher Education «Petrozavodsk State University»; Department of Hospital Therapyд.м.н., проф., каф. госпитальной терапии Петрозаводского государственного университета Petrozavodsk, Russia
References
- Bartel D. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004; 116:281-297. doi: http://dx.doi.org/10.1016/S0092-8674(04)00045-5
- Ceribelli A, Nahid M, Satoh M, Chan E. MicroRNAs in rheumatoid arthritis. FEBS letters. 2011; 585(23):3667-3674. doi: 10.1016/j.febslet.2011.05.020
- Баулина Н.М., Кулакова О.Г., Фаворова О.О. МикроРНК: роль в развитии аутоиммунного воспаления. Acta Naturae. 2016; 8(1): 23-35. http://elibrary.ru/item.asp?id=25790907
- Насонов Е.Л. Проблемы ревматоидного артрита в ХХI столетии. Вестник Российской академии наук. 2015;85(8): 744-754. doi: https://doi.org/10.7868/s0869587315080204
- Wittmann J, Jäck H. MicroRNAs in rheumatoid arthritis: midget RNAs with a giant impact. Annals of the rheumatic diseases. 2011; 70:92-96. doi: 10.1136/ard.2010.140152
- Alsaleh G, Nehmar R, Blüml S, Schleiss C, Ostermann E, Dillenseger J, Sayeh A, Choquet P, Dembele D, Francois A, Salmon J, Paul N, Schabbauer G, Bierry G, Meyer A, Gottenberg J, Haas G, Pfeffer S, Vallat L, Sibilia J, Bahram S, Georgel P. Reduced DICER1 Expression Bestows Rheumatoid Arthritis Synoviocytes Proinflammatory Properties and Resistance to Apoptotic Stimuli. Arthritis & rheumatology (Hoboken, NJ). 2016; 68(8):1839-1848. doi: 10.1002/art.39641
- Guo X, Liao Q, Chen P, Li X, Xiong W, Ma J, Li X, Luo Z, Tang H, Deng M, Zheng Y, Wang R, Zhang W, Li G. The microRNA-processing enzymes: Drosha and Dicer can predict prognosis of nasopharyngeal carcinoma. Journal of cancer research and clinical oncology. 2012; 138(1):49-56. doi: 10.1007/s00432-011-1058-1
- Nakamachi Y, Kawano S, Takenokuchi M, Nishimura K, Sakai Y, Chin T, Saura R, Kurosaka M, Kumagai S. MicroRNA-124a is a key regulator of proliferation and monocyte chemoattractant protein 1 secretion in fibroblast - like synoviocytes from patients with rheumatoid arthritis. Arthritis and rheumatism. 2009; 60:1294-1304. doi: 10.1002/art.24475
- Hruskova V, Jandova R, Vernerova L, Mann H, Pecha O, Prajzlerova K, Pavelka K, Vencovsky J, Filkova M, Senolt L. MicroRNA-125b: association with disease activity and the treatment response of patients with early rheumatoid arthritis. Arthritis research & therapy. 2016; 18(1):124. doi: 10.1186/s13075-016-1023-0
- Stanczyk J, Ospelt C, Karouzakis E, Filer A, Raza K, Kolling C, Gay R, Buckley C, Tak P, Gay S, Kyburz D. Altered expression of microRNA-203 in rheumatoid arthritis synovial fibroblasts and its role in fibroblast activation. Arthritis and rheumatism. 2011; 63:373-381. doi: 10.1002/art.30115
- Nakasa T, Miyaki S, Okubo A, Hashimoto M, Nishida K, Ochi M, Asahara H. Expression of microRNA-146 in rheumatoid arthritis synovial tissue. Arthritis and rheumatism. 2008; 58:1284-1292. doi:10.1002/ art.23429
- Pauley K, Satoh M, Chan A, Bubb M, Reeves W, Chan E. Upregulated miR-146a expression in peripheral blood mononuclearncells from rheumatoid arthritis patients. Arthritis research & therapy. 2008; 10(4):R101. doi: 10.1186/ar2493
- Saeki M, Watanabe M, Inoue N, Tokiyoshi E, Takuse Y, Arakawa Y, Hidaka Y, Iwatani Y. DICER and DROSHA gene expression and polymorphisms in autoimmune thyroid diseases. Autoimmunity. 2016; 49(8):514-522. doi: 10.1080/08916934.2016.1230846
- Magner W, Weinstock-Guttman B, Rho M, Hojnacki D, Ghazi R, Ramanathan M, Tomasi T. Dicer and microRNA expression in multiple sclerosis and response to interferon therapy. Journal of neuroimmunology. 2016; 292:68-78. doi: 10.1016/j.jneuroim.2016.01.009
- Aggarwal B. Tumour necrosis factors receptor associated signaling molecules and their role in activation of apoptosis, JNK and NF-kappaB. Ann Rheum Dis. 2000; 59:6-16. doi: 10.1136/ard.59.suppl_1.i6
- Guan Y, Yao H, Wang J, Sun K, Cao L, Wang Y. NF-κB-DICER-miRs Axis Regulates TNF-α Expression in Responses to Endotoxin Stress. International journal of biological sciences. 2015; 11(11):1257-1268. doi: 10.7150/ijbs.12611
- Малышева И.Е., Топчиева Л.В., Барышева О.Ю., Курбатова И.В., Васькова О.А., Везикова Н.Н., Марусенко И.М., член - корр. РАН Немова Н.Н. Содержание цитокинов и уровень экспрессии генов каспаз при ревматоидном артрите. Доклады Академии наук. 2016; 468(6):707-709. doi: 10.7868/S0869565216180262
- Andersson M, Turkkila M, Erlandsson M, Bossios A, Silfverswärd S, Hu D, Ekerljung L, Malmhäll C, Weiner H, Lundbäck B, Bokarewa M. Survivin controls biogenesis of microRNA in smokers: A link to pathogenesis of rheumatoid arthritis. Biochimica et biophysica acta. 2016; 1863(3):663-673. doi: 10.1016/j.bbadis.2016.11.033
- Spurlock C, Gass H, Bryant C, Wells B, Olsen N, Aune T. Methotrexate - mediated inhibition of nuclear factor κB activation by distinct pathways in T cells and fibroblast - like synoviocytes. Rheumatology (Oxford, England). 2015; 54(1):178-187. doi: 10.1093/rheumatology/keu279
- Kim S, Lee J, Nam S. Dicer Is Down - regulated and Correlated with Drosha in Idiopathic Sudden Sensorineural Hearing Loss. Journal of Korean medical science. 2015; 30(8):1183-1188. doi: 10.3346/jkms. 2015.30.8.1183
