电邮这篇文章 DICER and DROSHA gene expression in peripheral mononuclear blood cells from rheumatoid arthritis patients under methotrexate therapy
- 作者: Malysheva IE1, Topchieva LV1, Balan OV1, Marusenko IM2,3, Barysheva OY2,3
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隶属关系:
- Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, laboratory of genetics
- Federal State Budgetary Educational Institution of Higher Education «Petrozavodsk State University»
- Department of Hospital Therapy
- 期: 卷 90, 编号 5 (2018)
- 页面: 72-75
- 栏目: Editorial
- URL: https://journals.rcsi.science/0040-3660/article/view/32761
- DOI: https://doi.org/10.26442/terarkh201890572-75
- ID: 32761
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详细
The aim of this research was studying the level of DROSHA and DICER genes expression in peripheral mononuclear blood cells (PBMC) in rheumatoid arthritis (RA) patients under methotrexate therapy. Materials and methods. 82 people (from 45 to 70 years) enrolled in this study were divided into 3 groups (the first one - healthy donors (n=33 median age 49.93±1.87); the second group - rheumatoid arthritis patients without any therapy (n=15; median age 57.28±15.18) and the third group (n=34; median age 60.88±9.02) - rheumatoid arthritis patients who treated at least 4 weeks with methotrexate therapy (10-20 mg/week). The DICER and DROSHA genes expression level was determined by real-time PCR. Results. The number of DICER gene transcripts in PBMC in rheumatoid arthritis patients without therapy as in RA patients treated with methotrexate was reduced in comparison with the healthy donors (p<0.001 and p>0.05 respectively). The level of DROSHA gene expression in PBMC was not significantly different in all groups enrolled in this study (p>0.05). Conclusion. Our findings suggest that that the DICER gene expression level in perifheral mononuclear blood cells decreased with the development of rheumatoid arthritis. Methotrexate doesn’t influence on the mRNA level of this gene.
作者简介
I Malysheva
Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, laboratory of genetics
Email: i.e.malysheva@yandex.ru
с.н.с. лаб. генетики ИБ КарНЦ РАН Petrozavodsk, Russia
L Topchieva
Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, laboratory of geneticsв.н.с. лаб. генетики ИБ КарНЦ РАН Petrozavodsk, Russia
O Balan
Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, laboratory of geneticsс.н.с. лаб. генетики ИБ КарНЦ РАН Petrozavodsk, Russia
I Marusenko
Federal State Budgetary Educational Institution of Higher Education «Petrozavodsk State University»; Department of Hospital Therapyд.м.н., проф., каф. госпитальной терапии Петрозаводского государственного университета Petrozavodsk, Russia
O Barysheva
Federal State Budgetary Educational Institution of Higher Education «Petrozavodsk State University»; Department of Hospital Therapyд.м.н., проф., каф. госпитальной терапии Петрозаводского государственного университета Petrozavodsk, Russia
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