Analysis of VEGF-A/VEGFR1/VEGFR2 gene expression in patients with myelodysplastic syndrome

  • Authors: Kalitin NN1, Dudina GA2, Semochkin SV3, Karamysheva AF1
  • Affiliations:
    1. ФГБУ «Российский онкологический научный центр им. Н.Н. Блохина» Минздрава России
    2. ГБУЗ «Московский клинический научно-практический центр им. А.С. Логинова ДЗ города Москвы»
    3. ФГБОУ ВО «Российский национальный исследовательский медицинский университет им. Н.И. Пирогова» Минздрава России
  • Issue: Vol 89, No 7 (2017)
  • Pages: 39-44
  • Section: Editorial
  • URL: https://journals.rcsi.science/0040-3660/article/view/32263
  • DOI: https://doi.org/10.17116/terarkh201789739-44
  • ID: 32263

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Abstract

Aim. To assess the significance of gene expression of the vascular endothelial growth factor-A (VEGF-A) and its interacting receptors VEGFR1 and VEGFR2 as potential diagnostic and prognostic molecular markers in patients with myelodysplastic syndrome (MDS). Materials and methods. A real time polymerase chain reaction (RT-PCR) assay was used to investigate the gene expression of VEGF-A, VEGFR1, and VEGFR2 in the mononuclear cell fractions obtained from 24 patients with MDS. Results. The expression of the 3 genes was identified in all the patients examined. There was the highest expression level of the VEGF-A gene (p<0.0001), whereas the expression of the VEGFR1 gene was higher than that of the VEGFR2 gene (p<0.001). The expression of the VEGF-A gene proved to be higher in patients at a higher risk of acute leukemia and positively correlated with the expression levels of the VEGFR1 gene (p<0.05) rather than that of the VEGFR2 gene. At the same time, patients with higher VEGFR1 gene expression had significantly lower overall survival rates (r=–0.5; p<0.05). Patients with intermediate-2 or high-risk acute leukemia showed an increase in the average expression levels of VEGF-A and VEGFR1 and a reduction in VEGFR2 expression. Conclusion. This investigation revealed correlations between the number of blast cells in patients with MDS and the expression levels of the VEGF-A gene and between the overall survival of patients with MDS and the expression levels of the VEGFR1 gene rather than those of the VEGF-A and VEGFR2 genes.

About the authors

N N Kalitin

ФГБУ «Российский онкологический научный центр им. Н.Н. Блохина» Минздрава России

Москва, Россия

G A Dudina

ГБУЗ «Московский клинический научно-практический центр им. А.С. Логинова ДЗ города Москвы»

Москва, Россия

S V Semochkin

ФГБОУ ВО «Российский национальный исследовательский медицинский университет им. Н.И. Пирогова» Минздрава России

Москва, Россия

A F Karamysheva

ФГБУ «Российский онкологический научный центр им. Н.Н. Блохина» Минздрава России

Москва, Россия

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