Email this article Characteristics of the expression of the transcription factor pSTAT3 in asthma
- Authors: Mineev VN1, Lalaeva TM1
-
Affiliations:
- Первый Санкт-Петербургский государственный медицинский университет им. акад. И.П. Павлова Минздрава России
- Issue: Vol 89, No 3 (2017)
- Pages: 48-53
- Section: Editorial
- URL: https://journals.rcsi.science/0040-3660/article/view/32193
- DOI: https://doi.org/10.17116/terarkh201789348-53
- ID: 32193
Cite item
Full Text
Abstract
Aim. To investigate the transcription factor pSTAT3 in overweight asthmatics on a model of peripheral blood mononuclear cells at baseline and during recombinant leptin modulation. Materials and methods. A flow fluorometric assay was used according to the standard Bio-Plex protocol on a Bio-Plex immunoanalyzer (a flow fluorimeter) (Bio-Rad, USA) using xMAP technology at baseline and during modulation with recombinant leptin (Leptin, human, recombinant, expressed in a E. coli, Sigma, USA). Results. There was an obvious reduction in the level of the transcription factor pSTAT3 in patients with non-allergic asthma and an increase in that in patients with allergic asthma (AA). Recombinant leptin modulation of pSTAT3 levels caused their paradoxical decrease in both overweight women younger than 45 years of age with AA and in those with non-allergic asthma. Conclusion. The elevated level of the transcription factor pSTAT3 in AA is probably due to the overexpression of pSTAT3 in this group of patients. The paradoxical decrease in pSTAT3 levels in overweight women under 45 years of age with AA, which is similar in the non-allergic asthma group, can be explained by the enhanced expression of negative SOCS3 regulators and by leptin resistance.
Full Text
##article.viewOnOriginalSite##About the authors
V N Mineev
Первый Санкт-Петербургский государственный медицинский университет им. акад. И.П. Павлова Минздрава РоссииСанкт-Петербург, Россия
T M Lalaeva
Первый Санкт-Петербургский государственный медицинский университет им. акад. И.П. Павлова Минздрава РоссииСанкт-Петербург, Россия
References
- Akimoto T, Numata F, Tamura M. Abrogation of bronchial eosinophilic inflammation and airway hyperreactivity in signal transducers and activators of transcription (STAT) 6-deficient mice. J Exp Med. 1998;187(9):1537-1542.
- Минеев В.Н., Сорокина Л.Н. Экспрессия STAT6 в лимфоцитах периферической крови больных аллергической бронхиальной астмой. Медицинская иммунология. 2007;9(4-5):405-411.
- Stritesky GL, Rajarajeswari Muthukrishnan, Sarita Sehra, Ritobrata Goswami, Duy Pham, Jared Travers, Evelyn T, David E. The transcription factor STAT3 is required for T helper 2 cell development. Immunity. 2011;34(1):39-49. doi:10.1016
- Mari N, Mélanie Hercor, Sébastien Denanglaire, Oberdan Leo. The capacity of Th2 lymphocytes to deliver B-cell help requires expression of the transcription factor STAT3. Eur J Immunol. 2013;43(6):1489-1498. doi: 10.1002/eji.201242938
- Boyum A. Separation of leukocytes from blood and bone marrow. J Scan Clinical Lab Invest. 1968;21(Suppl.97):77.
- Sanches-Margalet V, Martin-Romero, Santos-Alvarez J, Goberna R, Najob S, Gonzalez-Yanes C. Role leptina as an immunomodulator of blood mononuclear cells: mechanisms of action. Clin Exp Immunol. 2003;133(1):11-19. doi: 10.1046/j.1365-2249
- Harrington LE, Hatton RD, Mangan PR, Henrietta Turner, Theresa L Murphy, Kenneth M Murphy, Casey T Weaver Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages. Nat Immunol. 2005;6(11):1123-1132. doi: 10.1038/ni1254
- Park H, Li Z, Yang XO. A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17. Nat Immunol. 2005;6(11):1133-1141. doi: 10.1038/ni1261
- Ishida-Takahashi R, Shigeo Uotani, Takahiro Abe, Mikako Degawa Yamauchi, Tetsuya Fukushima, Naruhiro Fujita, Hiroyuki Sakamaki, Hironori Yamasaki, Yoshihiko Yamaguchi, Katsumi Eguchi. Rapid inhibition of leptin signaling by glucocorticoids in vitro and in vivo. J Biol Chem. 2004;297(19):19658-19664. doi: 10.1074/jbc.M310864200
- Лим В.В., Сорокина Л.Н. Экспрессия негативных регуляторов транскрипции генов SОCS3 и SOCS5 в мононуклеарных клетках периферической крови больных бронхиальной астмой. Медицинская иммунология. 2014;2:149-154.
- Procaccini C, Elaine V Lourenco1, Giuseppe Matarese, Antonio La Cava. Leptin signaling: A key pathway in immune responses. Curr Signal Transduct Ther. 2009;4(1):22-30. doi: 10.2174/157436209787048711
- Xu D, Yin C, Wang S, Xiao Y. JAK-STAT in lipid metabolism of adipocytes. JAK-STAT. 2013;2(4). doi: 10.4161/jkst.27203
