Humoral and cellular adaptation mechanisms in development of intoxication syndrome in patients with acute viral hepatitis
- Authors: Рак SG1, Volchkova Y.V1, Umbetova КТ1
-
Affiliations:
- ММА им. И. М. Сеченова
- Issue: Vol 79, No 11 (2004)
- Pages: 61-65
- Section: Editorial
- URL: https://journals.rcsi.science/0040-3660/article/view/29947
- ID: 29947
Cite item
Full Text
Abstract
Aim. To study endotoxin-binding function of blood in patients with acute viral hepatitis (AVH) in the
course of the disease.
Material and methods. A total of 335 patients with AVH were examined. The titers of IgG antiendotoxin
antibodies to glycolipid of Re-chemotype were estimated in enzyme immunoassay (Diaendotox-2
kit), endotoxin-binding activity (EBA) of high density lipoproteins (HDLP) in EIA with the kit
Liprobind (Microecos). The number of polymorphonuclear leukocytes (PL) binding lipopolysaccharides
were counted by M. Yu. Yakovlev et al. method.
For detection of PL binding endotoxin of gram-negative bacteria, kits Endim (Microecos) were used,
beta2-microglobulin was estimated by a radioimmunoassay using kits made in Belarus Institute of
Bioorganic Chemistry. Registration of chemoluminescence was conducted on chemiluminometer LBK
1251 (Finland) by M. Wilson (1985) method.
Results. In moderate AVH at the height of intoxication a low Re-antibodies level was recorded irrespective
of the disease etiology. To convalescence this level went up. PL reserves in AVH patients were
low except patients with AVH С who had unaffected neutrophil reverves in relation to LPS binding. At
the height of AVH, HDLP EBA was very high in all the groups with normalization to convalescence.
In VHC high HDLP EBA persisted as long as the disease course. A significant rise of beta2-microglobulin
was seen in all the groups of AVH patients, its severity correlating with the degree of the
disease severity.
Conclusion. One of the leading factors in development of indoxication in AVH patients is a lipopolysaccharide
complex of gram-negative intestinal bacteria actively coming into blood flow due to hepatocyte
cytolysis. In response to LPS action there appeared a stereotypic adaptation complex of reactions
in the form of activation of cellular and humoral immune response.
course of the disease.
Material and methods. A total of 335 patients with AVH were examined. The titers of IgG antiendotoxin
antibodies to glycolipid of Re-chemotype were estimated in enzyme immunoassay (Diaendotox-2
kit), endotoxin-binding activity (EBA) of high density lipoproteins (HDLP) in EIA with the kit
Liprobind (Microecos). The number of polymorphonuclear leukocytes (PL) binding lipopolysaccharides
were counted by M. Yu. Yakovlev et al. method.
For detection of PL binding endotoxin of gram-negative bacteria, kits Endim (Microecos) were used,
beta2-microglobulin was estimated by a radioimmunoassay using kits made in Belarus Institute of
Bioorganic Chemistry. Registration of chemoluminescence was conducted on chemiluminometer LBK
1251 (Finland) by M. Wilson (1985) method.
Results. In moderate AVH at the height of intoxication a low Re-antibodies level was recorded irrespective
of the disease etiology. To convalescence this level went up. PL reserves in AVH patients were
low except patients with AVH С who had unaffected neutrophil reverves in relation to LPS binding. At
the height of AVH, HDLP EBA was very high in all the groups with normalization to convalescence.
In VHC high HDLP EBA persisted as long as the disease course. A significant rise of beta2-microglobulin
was seen in all the groups of AVH patients, its severity correlating with the degree of the
disease severity.
Conclusion. One of the leading factors in development of indoxication in AVH patients is a lipopolysaccharide
complex of gram-negative intestinal bacteria actively coming into blood flow due to hepatocyte
cytolysis. In response to LPS action there appeared a stereotypic adaptation complex of reactions
in the form of activation of cellular and humoral immune response.
About the authors
S G Рак
ММА им. И. М. СеченоваММА им. И. М. Сеченова
Ye V Volchkova
ММА им. И. М. СеченоваММА им. И. М. Сеченова
К Т Umbetova
ММА им. И. М. СеченоваММА им. И. М. Сеченова
References
- Пак С. Г., Малое В. А. Проблема синдрома интоксикации в инфекционной патологии. В кн.: Патогенез, иммунология, клиника и диагностика инфекционных болезней. М.: Медицина; 1992. 55-62.
- Покровский В. И., Бродов Л. Е., Малеев В. В. и др. Клиническая характеристика интоксикационного синдрома при салъмонеллезе. Тер. арх. 1983; 4: 138-141.
- Долин М. В., Фиш Н. Г. Белковые токсины микробов. М.: Медицина; 1981.
- Езепчук Ю. В. Патогенность как функция биомолекул. М.: Медицина; 1985.
- Fox E. S., Broitman S. A., Thomas P. Bacterial enodtoxins and the liver. Lab. Invest. 1990: 63 (6): 739-741.
- Яковлев М. Ю. Системная эндотоксинемия в физиологии и патологии человека. Автореф. дис. ... д-ра мед. наук. М.; 1993. 56 с.
- Лиходед В. Г., Яковлев М. Ю. Способ определения антиэндотоксинового иммунитета. А. с. № 9401519/14 ст. 18.04.94. Россия.
- Crawford J. M. Graft - versus-host disease of the liver. In:Farrara J. L. M., Deeg J., Burakoff S., eds. Graft-versus-host disease. New York: Dekker; 1996. 315-336.
- Пермяков Н. К., Аниховская В. А. Диагностика системной эндотоксинемии. В кн.: Яковлев М. Ю. (ред.) Сборник научн. трудов РАМН и Института морфологии человека. М.; 1993. 7-10.
- Muto G., Ohnishi H., Chisari F. V. Pathobiolodgy of fulminant hepatitis. In: Viral hepatitis and liver diseases. Tokyo. 1994. 200-203.
- Клеменко В. С., Титов В. Н. Бета-2-микроглобулин: структура, функции и клиническое значение. Лаб. дело 1984; 3: 131-137.
- Clement J. D., Chan S. Y., Dishop D. K. Allogenic class 1 MH requirement for alloantigen-reactive helper T-rymphocyte responses in vivo. Evidence for indirect presentation of alloantigen. Transplantation 1996; 62: 388-396.
- Владимиров Ю. А., Шерстнев М. П. Хемилюминесценшвд клеток животных. (Итоги науки и биотехники. Сер. Биофизика. Т. 24). М.; 1989.
- Шерлок Ш., Дули Дж. Заболевания печени и желчных путей. М.: ГЭОТАР Медицина; 1999.
- Пермяков Я. К., Аниховская И. А., Крупник А. Н. Иммуноморфологическая оценка резервов связывания эндотоксинов полиморфно-ядерными лейкоцитами. Арх. пат. 1995; 2: 4-7.
![](/img/style/loading.gif)