Email this article Humoral and cellular adaptation mechanisms in development of intoxication syndrome in patients with acute viral hepatitis
- Authors: Рак SG1, Volchkova Y.V1, Umbetova КТ1
-
Affiliations:
- ММА им. И. М. Сеченова
- Issue: Vol 79, No 11 (2004)
- Pages: 61-65
- Section: Editorial
- URL: https://journals.rcsi.science/0040-3660/article/view/29947
- ID: 29947
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Abstract
Aim. To study endotoxin-binding function of blood in patients with acute viral hepatitis (AVH) in the
course of the disease.
Material and methods. A total of 335 patients with AVH were examined. The titers of IgG antiendotoxin
antibodies to glycolipid of Re-chemotype were estimated in enzyme immunoassay (Diaendotox-2
kit), endotoxin-binding activity (EBA) of high density lipoproteins (HDLP) in EIA with the kit
Liprobind (Microecos). The number of polymorphonuclear leukocytes (PL) binding lipopolysaccharides
were counted by M. Yu. Yakovlev et al. method.
For detection of PL binding endotoxin of gram-negative bacteria, kits Endim (Microecos) were used,
beta2-microglobulin was estimated by a radioimmunoassay using kits made in Belarus Institute of
Bioorganic Chemistry. Registration of chemoluminescence was conducted on chemiluminometer LBK
1251 (Finland) by M. Wilson (1985) method.
Results. In moderate AVH at the height of intoxication a low Re-antibodies level was recorded irrespective
of the disease etiology. To convalescence this level went up. PL reserves in AVH patients were
low except patients with AVH С who had unaffected neutrophil reverves in relation to LPS binding. At
the height of AVH, HDLP EBA was very high in all the groups with normalization to convalescence.
In VHC high HDLP EBA persisted as long as the disease course. A significant rise of beta2-microglobulin
was seen in all the groups of AVH patients, its severity correlating with the degree of the
disease severity.
Conclusion. One of the leading factors in development of indoxication in AVH patients is a lipopolysaccharide
complex of gram-negative intestinal bacteria actively coming into blood flow due to hepatocyte
cytolysis. In response to LPS action there appeared a stereotypic adaptation complex of reactions
in the form of activation of cellular and humoral immune response.
course of the disease.
Material and methods. A total of 335 patients with AVH were examined. The titers of IgG antiendotoxin
antibodies to glycolipid of Re-chemotype were estimated in enzyme immunoassay (Diaendotox-2
kit), endotoxin-binding activity (EBA) of high density lipoproteins (HDLP) in EIA with the kit
Liprobind (Microecos). The number of polymorphonuclear leukocytes (PL) binding lipopolysaccharides
were counted by M. Yu. Yakovlev et al. method.
For detection of PL binding endotoxin of gram-negative bacteria, kits Endim (Microecos) were used,
beta2-microglobulin was estimated by a radioimmunoassay using kits made in Belarus Institute of
Bioorganic Chemistry. Registration of chemoluminescence was conducted on chemiluminometer LBK
1251 (Finland) by M. Wilson (1985) method.
Results. In moderate AVH at the height of intoxication a low Re-antibodies level was recorded irrespective
of the disease etiology. To convalescence this level went up. PL reserves in AVH patients were
low except patients with AVH С who had unaffected neutrophil reverves in relation to LPS binding. At
the height of AVH, HDLP EBA was very high in all the groups with normalization to convalescence.
In VHC high HDLP EBA persisted as long as the disease course. A significant rise of beta2-microglobulin
was seen in all the groups of AVH patients, its severity correlating with the degree of the
disease severity.
Conclusion. One of the leading factors in development of indoxication in AVH patients is a lipopolysaccharide
complex of gram-negative intestinal bacteria actively coming into blood flow due to hepatocyte
cytolysis. In response to LPS action there appeared a stereotypic adaptation complex of reactions
in the form of activation of cellular and humoral immune response.
About the authors
S G Рак
ММА им. И. М. СеченоваММА им. И. М. Сеченова
Ye V Volchkova
ММА им. И. М. СеченоваММА им. И. М. Сеченова
К Т Umbetova
ММА им. И. М. СеченоваММА им. И. М. Сеченова
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