Molecular mechanisms of the effect of standardized placental hydrolysate peptides on mitochondria functioning
- Authors: Torshin I.Y.1, Gromova O.A.1, Tikhonova O.V.2, Chuchalin A.G.3
-
Affiliations:
- Computer Science and Control
- Orekhovich Research Institute of Biomedical Chemistry
- Pirogov Russian National Research Medical University
- Issue: Vol 95, No 12 (2023)
- Pages: 1133-1140
- Section: Original articles
- URL: https://journals.rcsi.science/0040-3660/article/view/258741
- DOI: https://doi.org/10.26442/00403660.2023.12.202494
- ID: 258741
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Abstract
Background. Human placenta hydrolysates (HPH), the study of which was initiated by the scientific school of Vladimir P. Filatov, are currently being investigated using modern proteomic technologies. HPH is a promising tool for maintaining the function of mitochondria and regenerating tissues and organs with a high content of mitochondria (liver, heart muscle, skeletal muscles, etc.). The molecular mechanisms of action of HPH are practically not studied.
Aim. Identification of mitochondrial support mitochondrial function-supporting peptides in HPH (Laennec, produced by Japan Bioproducts).
Materials and methods. Data on the chemical structure of the peptides were collected through a mass spectrometric experiment. Then, to establish the amino acid sequences of the peptides, de novo peptide sequencing algorithms based on the mathematical theory of topological and metric analysis of chemographs were applied. Bioinformatic analysis of the peptide composition of HPH was carried out using the integral protein annotation method.
Results. The biological functions of 41 peptides in the composition of HPH have been identified and described. Among the target proteins, the activity of which is regulated by the identified peptides and significantly affects the function of mitochondria, are caspases (CASP1, CASP3, CASP4) and other proteins regulating apoptosis (BCL2, CANPL1, PPARA), MAP kinases (MAPK1, MAPK3, MAPK4, MAPK8, MAPK9 , MAPK10, MAPK14), AKT1/GSK3B/MTOR cascade kinases, and a number of other target proteins (ADGRG6 receptor, inhibitor of NF-êB kinase IKKE, pyruvate dehydrogenase 2/3/4, SIRT1 sirtuin deacetylase, ULK1 kinase).
Conclusion. HPH peptides have been identified that promote inhibition of mitochondrial pore formation, apoptosis, and excessive mitochondrial autophagy under conditions of oxidative/toxic stress, chronic inflammation, and/or hyperinsulinemia.
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##article.viewOnOriginalSite##About the authors
Ivan Yu. Torshin
Computer Science and Control
Email: unesco.gromova@gmail.com
ORCID iD: 0000-0002-2659-7998
канд. физ.-мат. наук, канд. хим. наук, вед. науч. сотр. ФИЦ ИУ РАН
Russian Federation, MoscowOlga A. Gromova
Computer Science and Control
Author for correspondence.
Email: unesco.gromova@gmail.com
ORCID iD: 0000-0002-7663-710X
Scopus Author ID: 7003589812
д-р мед. наук, проф., вед. науч. сотр. ФИЦ ИУ РАН
Russian Federation, MoscowOlga V. Tikhonova
Orekhovich Research Institute of Biomedical Chemistry
Email: unesco.gromova@gmail.com
ORCID iD: 0000-0002-2810-566X
канд. биол. наук, рук. Центра коллективного пользования «Протеом человека» ФГБНУ «НИИБМХ им. В.Н. Ореховича»
Russian Federation, MoscowAlexander G. Chuchalin
Pirogov Russian National Research Medical University
Email: unesco.gromova@gmail.com
ORCID iD: 0000-0002-6808-5528
акад. РАН, д-р мед. наук, проф., зав. каф. госпитальной терапии педиатрического фак-та, председатель правления Российского респираторного общества
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