Carbohydrase activity in proximal and distal segments of small intestine in health, irritated bowel syndrome and enteropathy with membrane digestion disturbance

Cover Page

Cite item

Full Text

Abstract

Diagnostics of enteropathy with membrane digestion disturbance (EMDD) is based on the evaluation of carbohydrase activity in duodenal mucosa (DM). There is no information about diagnostic value of ileum mucosa (IM) carbohydrases.

Aim. Assess the diagnostic value of carbohydrases activity in mucous membrane of duodenum and distal ileum in patients with EMDD and irritable bowel syndrome (IBS).

Materials and methods. The study includes 12 patients with EMDD, 16 patients with IBS and 12 relatively healthy persons (control group). Study design included analysis of food tolerance and evaluation of activity of sucrase, glucoamylase, maltase and lactase in mucous membrane of duodenum and ileum based on the method of Dahlquist with N.I. Belostotsky modification. Microsoft Office Excel and Statistica 10.0 were used for statistical evaluation of material. Mann–Whitney criterion was used to evaluate the differences between quantitative parameters. The differences were considered to be significant at p<0.05. Control group was used for comparison.

Results. In control group the carbohydrases activity in duodenum mucosa was not significantly statistically different from the one in IM. In patients of the IBS group, the activity of glucoamylase and maltase in the IM was significantly higher than in the duodenum: glucoamylase by 2.2 times, maltase by 2 times. In patients with EMDD the activity of glucoamylase, maltase and sucrase in IM is higher than in DM by 5.2, 3.8 and 2 times respectively, which signifies the formation of compensatory reaction of adaptive shift, initially described by A.M. Ugolev in 1985.

Conclusion. The evaluation of carbohydrase activity in IM provides more information about pathogenesis of EMDD and its differential diagnosis with IBS.

About the authors

Elena A. Kondrashova

LLC «Yauza Medical Center»

Author for correspondence.
Email: kondrashova@yamed.ru
ORCID iD: 0009-0004-6754-849X

врач – гастроэнтеролог-эндоскопист

Russian Federation, Moscow

Nickolai I. Belostotsky

Loginov Moscow Clinical Scientific Center

Email: kondrashova@yamed.ru
ORCID iD: 0000-0003-4400-1227

д-р мед. наук, ст. науч. сотр. лаб. доклинических методов исследования 

Russian Federation, Moscow

Asfold I. Parfenov

Loginov Moscow Clinical Scientific Center

Email: kondrashova@yamed.ru

д-р мед. наук, проф., зав. отд. патологии кишечника 

Russian Federation, Moscow

References

  1. Погромов А.П., Мнацаканян М.Г., Тащян О.В. Распространенность синдрома раздраженного кишечника. Клиническая медицина. 2016;94(11):869-74 [Pogromov AP, Mnatsakanyan MG, Tashyan OV. Prevalence of irritable bowel syndrome. Clinical Medicine, Russian Journal. 2016;94(11):869-74 (in Russian)]. doi: 10.18821/0023-2149-2016-94-11-869-874
  2. Lacy BE, Pimentel M, Brenner DM, et al. ACG Clinical Guideline: Management of irritable bowel syndrome. Am J Gastroenterol. 2021;116(1):17-44. doi: 10.14309/ajg.0000000000001036
  3. Маев И.В., Черемушкин С.В., Кучерявый Ю.А., Черемушкина Н.В. Алгоритм ведения пациентов с синдромом раздраженного кишечника. Римские критерии IV. РМЖ. 2017;10:718-22 [Maev IV, Cheryomushkin SV, Kucheryavy YuA, Cheryomushkina NV. Algorithm for managing patients with irritable bowel syndrome. Roman criteria IV. RMJ. 2017;10:718-22 (in Russian)].
  4. Парфенов А.И. Пищевая непереносимость и тонкая кишка. Терапевтический архив. 2022;94(2):153-9 [Parfenov AI. Food intolerance and small intestine. Terapevticheskii Arkhiv (Ter. Arkh.). 2022;94(2):153-9 (in Russian)]. doi: 10.26442/00403660.2022.02.201364
  5. Dugum M, Barco K, Garg S. Managing irritable bowel syndrome: The low-FODMAP diet. Cleve Clin J Med. 2016;83(9):655-62. doi: 10.3949/ccjm.83a.14159
  6. Парфенов А.И., Ахмадуллина О.В., Сабельникова Е.А., и др. Дисахаридазная недостаточность под маской функционального заболевания кишечника. Фарматека. 2019;26(2):48-53 [Parfenov AI, Akhmadullina OV, Sabelnikova EA, et al. Disaccharidase deficiency under the guise of functional bowel disease. Pharmateka. 2019;26(2):48-53 (in Russian)]. doi: 10.18565/pharmateca.2019.2.48-53
  7. Парфенов А.И., Ахмадуллина О.В., Белостоцкий Н.И., и др. Дисахаридазная недостаточность – одна из причин функциональных заболеваний кишечника. Клиническая медицина. 2018;96(4):365-70 [Parfenov АI, Ahmadullina OV, Belostoskij NI, et al. Disaharidase deficiency – is one of the causes of functional disorders of intestine. Clinical Medicine, Russian Journal. 2018;96(4):365-70 (in Russian)]. doi: 10.18821/0023-2149-2018-96-4-365-370
  8. Burke M. Carbohydrate intolerance and disaccharidase measurement – A mini-review. Clin Biochem Rev. 2019;40(4):167-74. doi: 10.33176/AACB-19-00025
  9. Парфенов А.И., Белостоцкий Н.И., Хомерики С.Г., и др. Ребамипид повышает активность карбогидраз у больных энтеропатией с нарушением мембранного пищеварения. Пилотное исследование. Терапевтический архив. 2019;91(2):25-31 [Parfenov АI, Belostotsky NI, Khomeriki SG, et al. Rebamipide increases the disaccharidases activity in patients with enteropathy with impaired membrane digestion. Pilot study. Terapevticheskii Arkhiv (Ter. Arkh.). 2019;91(2):25-31 (in Russian)]. doi: 10.26442/00403660.2019.02.000123
  10. Бауло Е.В., Белостоцкий Н.И., Ахмадуллина О.В., и др. Влияние цитопротектора ребамипида на активность карбогидраз у больных энтеропатией с нарушением мембранного пищеварения. Эффективная фармакотерапия. 2021;17(28):10-4 [Baulo EV, Belostotsky NI, Akhmadullina OV, et al. The effect of the cytoprotector rebamipid on the activity of disaccharidases in patients with enteropathy with impaired membrane digestion. Effective Pharmacotherapy. 2021;17(28):10-4 (in Russian)]. doi: 10.33978/2307-3586-2021-17-28-10-14
  11. Парфенов А.И., Сабельникова Е.А., Быкова С.В., и др. Энтеропатия с нарушением мембранного пищеварения как нозологическая форма. Медицинский алфавит. 2019;1(6):37-46 [Parfenov AI, Sabelnikova EA, Bykova SV, et al. Enteropathy with impaired membrane digestion as nosological form. Medical Alphabet. 2019;1(6):37-46 (in Russian)]. doi: 10.33667/2078-5631-2019-1-6(381)-37-46
  12. Hackenmueller SA, Grenache DG. Reference intervals for intestinal disaccharidase activities determined from a non-reference population. J Appl Lab Med. 2016;1(2):172-80. doi: 10.1373/jalm.2016.020388
  13. Белостоцкий Н.И., Новиков А.А., Парфенов А.И. Модификация метода исследования активности карбогидраз в биоптатах слизистой оболочки тонкой кишки. Справочник заведующего КДЛ. 2021;3:73-9 [Belostotsky NI, Novikov AA, Parfenov AI. Modification of the method for studying the activity of carbohydrases in biopsy specimens of the small intestine mucosa. Directory of the Head of the CDL. 2021;3:73-9 (in Russian)].
  14. Ганусенкова М.И. А.М. Уголев, вклад в мировую науку. Образование и наука в России и за рубежом. 2018;7(42):116-9 [Ganusenkova MI. A.M. Ugolev, contribution to world science. Education and Science in Russia and Abroad. 2018;7(42):116-9 (in Russian)].
  15. Puertolas MV, Fifi AC. The role of disaccharidase deficiencies in functional abdominal pain disorders – A narrative review. Nutrients. 2018;10(12):1835. doi: 10.3390/nu10121835
  16. Amiri M, Naim HY. Characterization of mucosal disaccharidases from human intestine. Nutrients. 2017;9(10):1106. doi: 10.3390/nu9101106
  17. Парфенов А.И. Энтеропатия с нарушением мембранного пищеварения. 2-е изд. М.: Медконгресс, 2022 [Parfenov AI. Enteropatiia s narusheniem membrannogo pishchevareniia. 2-e izd. Moscow: Medkongress, 2022 (in Russian)].
  18. Уголев А.М. Эволюция пищеварения и принципы эволюции функций. Л.: Наука, 1985 [Ugolev AM. Evoliutsiia pishchevareniia i printsipy evoliutsii funktsii. Leningrad: Nauka, 1985 (in Russian)].

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Fig. 1. The ileum/duodenum ratio of maltase activity (%).

Download (49KB)
Indexing metadata
3. Fig. 2. The ileum/duodenum ratio of glucoamylase activity (%).

Download (62KB)
Indexing metadata
4. Fig. 3. The ileum/duodenum ratio of sucrase activity (%).

Download (51KB)
Indexing metadata
5. Fig. 4. The ileum/duodenum ratio of lactase activity (%).

Download (53KB)
Indexing metadata

Copyright (c) 2024 Consilium Medicum

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
 
 


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies