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Polysaccharides of Crotalaria alata and Gleditsia triacanthos as Components of a Growth Medium for Cultivation of Microfungi
- Authors: Zakirova R.P.1, Kodiralieva F.1, Hwan A.M.1, Rakhmanberdieva R.K.1
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Affiliations:
- Acad. Yunusov Institute of Chemistry of the Plant Substances, AS RUz
- Issue: Vol 59, No 3 (2023)
- Pages: 306-313
- Section: Component Composition of Resource Species
- URL: https://journals.rcsi.science/0033-9946/article/view/138806
- DOI: https://doi.org/10.31857/S003399462303007X
- EDN: https://elibrary.ru/SBYVAM
- ID: 138806
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Abstract
Abstract
—The study explores the possibility of using the seeds of Crotalaria alata L. and Gleditsia triacanthos L. (Fabaceae) as a growth medium stabilizer for cultivation of microfungi. A synergistic effect was achieved when agar-agar was combined with galactomannans from Crotalaria alata and Gleditsia triacanthos, in a ratio of 1.5 : 4 : 1. Such a composition made it possible to obtain a solid growth medium, similar in consistency and color to 2% agar-agar medium. To establish biological parameters of the developed medium, the spores of microfungi Stachybotrys alternans Bonord. (Stachybotrys Cordа) и Fusarium oxysporum Schr f. vasinfectum Bilai (Fusarium), were plated on the surface of the growth media using inoculation needle and incubated in a thermostat at 25–26 °C. It was shown that partial substitution of agar-agar with galactomannans contributes to the active growth of the microorganisms. The growth of the Stachybotrys alternans and Fusarium oxysporum fungal colonies on the galactomannans growth medium surpassed the control on the fifth day by 37.2 and 31.5%, and on the seventh day – by 8.7 and 11.1% respectively.
About the authors
R. P. Zakirova
Acad. Yunusov Institute of Chemistry of the Plant Substances, AS RUz
Author for correspondence.
Email: ranozakirova@mail.ru
Republic of Uzbekistan, Tashkent
F. Kodiralieva
Acad. Yunusov Institute of Chemistry of the Plant Substances, AS RUz
Email: ranozakirova@mail.ru
Republic of Uzbekistan, Tashkent
A. M. Hwan
Acad. Yunusov Institute of Chemistry of the Plant Substances, AS RUz
Email: ranozakirova@mail.ru
Republic of Uzbekistan, Tashkent
R. K. Rakhmanberdieva
Acad. Yunusov Institute of Chemistry of the Plant Substances, AS RUz
Email: ranozakirova@mail.ru
Republic of Uzbekistan, Tashkent
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