The first Genetic data on Lernaea Cyprinacea Linnaeus, 1758 from the European North of Russia (The Pinega River)

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Abstract

Morphological and molecular genetic data about Lernaea cyprinacea Linnaeus, 1758, collected from pike (Esox lucius Linnaeus, 1758) in the European North of Russia (Pinega river) in 2022, were presented. Molecular genetic studies were carried out based on the analysis of COI mtDNA, 18S rDNA and 28S rDNA gene sequences. A significant genetic distance for the COI gene from individuals of the same species collected in other territories (China, Australia, South Africa, Canada, Austria, Argentina, Pakistan, Iran, India, Japan, Romania, Egypt and Western Siberia) was revealed, which indicates the existence of a cryptic species L. cyprinacea in the European North of Russia. Analysis of 28S rDNA sequences showed the presence of two substitutions fixed in the population, which may indicate a fairly long-standing divergence of sister species. The 18S rDNA gene fragment is less suitable for identifying L. cyprinacea due to low variability compared to the other two gene regions.

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I. A. Kuznetsova

ФГБУН Федеральный исследовательский центр комплексного изучения Арктики имени академика Н.П. Лаверова УрО РАН

Author for correspondence.
Email: kia.iepn@gmail.com
Russian Federation, пр. Никольский, 20, Архангельск, 163020

A. V. Kondakov

ФГБУН Федеральный исследовательский центр комплексного изучения Арктики имени академика Н.П. Лаверова УрО РАН

Email: kia.iepn@gmail.com
Russian Federation, пр. Никольский, 20, Архангельск, 163020

T. A. Eliseeva

ФГБУН Федеральный исследовательский центр комплексного изучения Арктики имени академика Н.П. Лаверова УрО РАН

Email: kia.iepn@gmail.com
Russian Federation, пр. Никольский, 20, Архангельск, 163020

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Supplementary files

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2. Figure 1. Pike with an attached individual of Lernaea cyprinacea L., 1758, one individual was removed.

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3. Figure 2. General view of Lernaea cyprinacea L., 1758 female: A – Female fixed in 96% ethanol; Б – Cephalothorax with the attachment apparatus after treatment with lactic acid, dorsum view; В – Individual after removal of the egg sacs and treatment with lactic acid. Scale bar 2.5 cm.

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4. Figure 3. Cephalothorax appendages of female Lernaea cyprinacea L., 1758: А – Oral apparatus; Б –Antennule; В – Antenna; Г – Maxilla II; Д – Maxilleped with seta (st); Е – Mandible. Scale bar 20 µm.

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5. Figure 4. 1–6 pairs of legs and furcal rami of Lernaea cyprinacea L., 1758: A – First pair of legs; Б – Second pair of legs; В – Fragment of a leg of the third pair; Г – Leg of the fourth pair; Д – Fifth pair of legs; Е – Sixth pair of legs; Ж – Furcal rami. Scale bar, µm: А–Д, Ж – 20; Е – 10.

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6. Figure 5. Maximum likelihood phylogenetic tree of the genus Lernaea based on COI. The scale bar shows the percentage sequence divergence of individual species superimposed on branch lengths. The numbers next to the nodes represent the bootstrap support values.

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