Email this article The Development of a Multiparametric Immunochromatographic Test System for the Analysis of Cardiomarkers
- Authors: Andreeva I.P.1, Yakovleva E.A.1, Grigorenko V.G.1, Osipov A.P.1,2
-
Affiliations:
- Department of Chemistry, Moscow State University
- National Research Technological University MISiS
- Issue: Vol 74, No 6 (2019)
- Pages: 315-321
- Section: Article
- URL: https://journals.rcsi.science/0027-1314/article/view/164068
- DOI: https://doi.org/10.3103/S002713141906004X
- ID: 164068
Cite item
Open Access
Access granted
Subscription Access
Abstract
Abstract—An immunochromatographic test system for the combined analysis of two cardiomarkers, such as fatty acid binding protein (FABP) and troponin I (TnI), in serum and whole blood is developed. The main stages of the design of a multiparametric system are investigated, including the influence of the antibody position and gold nanoparticle size on the results. The detection limits of the method are found to be 10 ng/mL for FABP and 1 ng/mL for TnI, and the coefficient of variation did not exceed 10%. The developed test system allows us to confirm the diagnosis of acute myocardial infraction (N = 9). The analysis time is 15 min.
About the authors
I. P. Andreeva
Department of Chemistry, Moscow State University
Email: aposipov@mail.ru
Russian Federation, Moscow
E. A. Yakovleva
Department of Chemistry, Moscow State University
Email: aposipov@mail.ru
Russian Federation, Moscow
V. G. Grigorenko
Department of Chemistry, Moscow State University
Email: aposipov@mail.ru
Russian Federation, Moscow
A. P. Osipov
Department of Chemistry, Moscow State University; National Research Technological University MISiS
Author for correspondence.
Email: aposipov@mail.ru
Russian Federation, Moscow; Moscow
