The Development of a Multiparametric Immunochromatographic Test System for the Analysis of Cardiomarkers
- Autores: Andreeva I.1, Yakovleva E.1, Grigorenko V.1, Osipov A.1,2
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Afiliações:
- Department of Chemistry, Moscow State University
- National Research Technological University MISiS
- Edição: Volume 74, Nº 6 (2019)
- Páginas: 315-321
- Seção: Article
- URL: https://journals.rcsi.science/0027-1314/article/view/164068
- DOI: https://doi.org/10.3103/S002713141906004X
- ID: 164068
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Resumo
Abstract—An immunochromatographic test system for the combined analysis of two cardiomarkers, such as fatty acid binding protein (FABP) and troponin I (TnI), in serum and whole blood is developed. The main stages of the design of a multiparametric system are investigated, including the influence of the antibody position and gold nanoparticle size on the results. The detection limits of the method are found to be 10 ng/mL for FABP and 1 ng/mL for TnI, and the coefficient of variation did not exceed 10%. The developed test system allows us to confirm the diagnosis of acute myocardial infraction (N = 9). The analysis time is 15 min.
Sobre autores
I. Andreeva
Department of Chemistry, Moscow State University
Email: aposipov@mail.ru
Rússia, Moscow
E. Yakovleva
Department of Chemistry, Moscow State University
Email: aposipov@mail.ru
Rússia, Moscow
V. Grigorenko
Department of Chemistry, Moscow State University
Email: aposipov@mail.ru
Rússia, Moscow
A. Osipov
Department of Chemistry, Moscow State University; National Research Technological University MISiS
Autor responsável pela correspondência
Email: aposipov@mail.ru
Rússia, Moscow; Moscow