DNA Sequence-Specific Ligands. XIX. Synthesis, Spectral Properties, Virological and Biochemical Studies of Fluorescent Dimeric Trisbenzimidazoles DB 3 ( n )

A. F. Arutyunyan; Арутюнян А. Ф.; A. A. Kostyukov; Костюков А. А.; S. P. Korolev; Королёв С. П.; M. B. Gottikh; Готтих М. Б.; O. Yu. Susova; Сусова О. Ю.; D. N. Kaluzhny; Калюжный Д. Н.; A. L. Zhuze; Жузе А. Л.

doi:10.31857/S0026898423030023

DNA Sequence-Specific Ligands. XIX. Synthesis, Spectral Properties, Virological and Biochemical Studies of Fluorescent Dimeric Trisbenzimidazoles DB₃(n)

Autores: Arutyunyan A.¹, Kostyukov A.², Korolev S.³^,4, Gottikh M.³^,4, Susova .⁵, Kaluzhny D.¹, Zhuze A.¹
Afiliações:
1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
2. Emanuel Institute of Biochemical Physics, Russian Academy of Science
3. Department of Chemistry, Lomonosov Moscow State University
4. Belozersky Institute of Physicochemical Biology, Lomonosov Moscow State University
5. Research Institute of Carcinogenesis, Blokhin National Medical Research Center of Oncology
Edição: Volume 57, Nº 3 (2023)
Páginas: 517-527
Seção: СТРУКТУРНО-ФУНКЦИОНАЛЬНЫЙ АНАЛИЗ БИОПОЛИМЕРОВ И ИХ КОМПЛЕКСОВ
URL: https://journals.rcsi.science/0026-8984/article/view/138638
DOI: https://doi.org/10.31857/S0026898423030023
EDN: https://elibrary.ru/CGXJLT
ID: 138638

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Resumo

In this work, we synthesized and characterized the properties of a series of new fluorescent narrow-groove ligands DB₃(n). DB₃(n) compounds based on dimeric trisbenzimidazoles have the ability to bind to the AT regions of DNA. The synthesis of DB₃(n), trisbenzimidazole fragments of which are linked by oligomethylene linkers of different lengths (n = 1, 5, 9), is based on the condensation of monomeric trisbenzimidazole MB₃ with α,ω-alkyldicarboxylic acids. DB₃(n) proved to be effective inhibitors of the catalytic activity of HIV-1 integrase at submicromolar concentrations (0.20–0.30 µM). DB₃(n) was found to inhibit the catalytic activity of DNA topoisomerase I at low micromolar concentrations.

Palavras-chave

DNA, sequence-specific minor-groove ligand, fluorescent dimeric trisbenzimidazoles DB₃(n), spectral methods, HIV-1 integrase, DNA topoisomerase I

Bibliografia

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