The Thymic Hormone Thymosin-1 α Reduces the Pro-Inflammatory Response of RAW 264.7 Cells Induced by Endotoxin

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The aim of this work was to study effects of thymosin-1 alpha (Tα1) on the anti-inflammatory response of RAW 264.7 macrophages cultured in the presence of lipopolysaccharide (LPS) from the walls of gram-negative bacteria. Also we evaluated production of pro-inflammatory cytokines and activity of the NF-κB and SAPK/JNK signaling pathways. In addition, the level of expression of a number of genes that regulate cell apoptosis, as well as the activity of receptors involved in the pro-inflammatory response, was determined. Firstly, the addition of Tα1 normalized the level of cytokine production to varying degrees, with a particularly noticeable effect on IL-1β and IL-6. Secondly, the addition of Tα1 normalized activity of the NF-κB and SAPK/JNK signaling cascades and the expression of the Tlr4 gene. Thirdly, Tα1 significantly reduced p53 and the activity of the P53 gene, which is a marker of cell apoptosis. Fourthly, it was shown that an increase in Ar-1 gene expression under the influence of LPS was significantly reduced using Tα1. Thus, it was found that the presence of Tα1 in the RAW 264.7 cell culture medium significantly reduced the level of the pro-inflammatory response of cells.

作者简介

E. Novoselova

Institute of Cell Biophysics, Russian Academy of Sciences

编辑信件的主要联系方式.
Email: elenanov_06@mail.ru
Russia, 142290, Moscow Region, Pushchino

O. Glushkova

Institute of Cell Biophysics, Russian Academy of Sciences

Email: elenanov_06@mail.ru
Russia, 142290, Moscow Region, Pushchino

M. Khrenov

Institute of Cell Biophysics, Russian Academy of Sciences

Email: elenanov_06@mail.ru
Russia, 142290, Moscow Region, Pushchino

S. Lunin

Institute of Cell Biophysics, Russian Academy of Sciences

Email: elenanov_06@mail.ru
Russia, 142290, Moscow Region, Pushchino

M. Sharapov

Institute of Cell Biophysics, Russian Academy of Sciences

Email: elenanov_06@mail.ru
Russia, 142290, Moscow Region, Pushchino

R. Goncharov

Institute of Cell Biophysics, Russian Academy of Sciences

Email: elenanov_06@mail.ru
Russia, 142290, Moscow Region, Pushchino

E. Mubarakshina

Institute of Cell Biophysics, Russian Academy of Sciences

Email: elenanov_06@mail.ru
Russia, 142290, Moscow Region, Pushchino

T. Novoselova

Institute of Cell Biophysics, Russian Academy of Sciences

Email: elenanov_06@mail.ru
Russia, 142290, Moscow Region, Pushchino

S. Parfenyuk

Institute of Cell Biophysics, Russian Academy of Sciences

Email: elenanov_06@mail.ru
Russia, 142290, Moscow Region, Pushchino

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版权所有 © Е.Г. Новоселова, О.В. Глушкова, М.О. Хренов, С.М. Лунин, М.Г. Шарапов, Р.Г. Гончаров, Э.К. Мубаракшина, Т.В. Новоселова, С.Б. Парфенюк, 2023

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