Methylation of Regulatory Regions of DNA Repair System Genes in Carotid Atherosclerosis
- Авторлар: Babushkina N.1, Zarubin A.1, Koroleva I.1, Gomboeva D.1, Bragina E.1, Goncharova I.1, Golubenko M.1, Salakhov R.1, Sleptcsov A.1, Kuznetsov M.2, Kozlov B.2, Muslimova E.2, Afanasiev S.2, Kucher A.1, Nazarenko M.1
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Мекемелер:
- Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
- Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences
- Шығарылым: Том 57, № 4 (2023)
- Беттер: 647-664
- Бөлім: ГЕНОМИКА. ТРАНСКРИПТОМИКА
- URL: https://journals.rcsi.science/0026-8984/article/view/138706
- DOI: https://doi.org/10.31857/S002689842304002X
- EDN: https://elibrary.ru/QKQNQS
- ID: 138706
Дәйексөз келтіру
Аннотация
The status of DNA methylation in the human genome changes during the pathogenesis of common diseases and acts as a predictor of life expectancy. Therefore, it is of interest to investigate the methylation level of regulatory regions of genes responsible for general biological processes that are potentially significant for the development of age-associated diseases. Among them there are genes encoding proteins of DNA repair system, which are characterized by pleiotropic effects. Here, results of the targeted methylation analysis of two regions of the human genome (the promoter of the MLH1 gene and the enhancer near the ATM gene) in different tissues of patients with carotid atherosclerosis are present. Analysis of the methylation profiles of studied genes in various tissues of the same individuals demonstrated marked differences between leukocytes and tissues of the vascular wall. Differences in methylation levels between normal and atherosclerotic tissues of the carotid arteries were revealed only for two studied CpG sites (chr11:108089866 and chr11:108090020, GRCh37/hg19 assembly) in the ATM gene. Based on this, we can assume the involvement of ATM in the development of atherosclerosis. “Overload” of the studied regions with transcription factor binding sites (according to ReMapp2022 data) indicate that the tissue-specific nature of methylation of the regulatory regions of the MLH1 and ATM may be associated with expression levels of these genes in a particular tissue. It has been shown that inter-individual differences in the methylation levels of CpG sites are associated with sufficiently distant nucleotide substitutions.
Негізгі сөздер
Авторлар туралы
N. Babushkina
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: nad.babushkina@medgenetics.ru
Russia, 634050, Tomsk
A. Zarubin
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634050, Tomsk
Iu. Koroleva
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634050, Tomsk
D. Gomboeva
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634050, Tomsk
E. Bragina
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634050, Tomsk
I. Goncharova
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634050, Tomsk
M. Golubenko
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634050, Tomsk
R. Salakhov
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634050, Tomsk
A. Sleptcsov
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634050, Tomsk
M. Kuznetsov
Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634034, Tomsk
B. Kozlov
Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634034, Tomsk
E. Muslimova
Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634034, Tomsk
S. Afanasiev
Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634034, Tomsk
A. Kucher
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634050, Tomsk
M. Nazarenko
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: nad.babushkina@medgenetics.ru
Russia, 634050, Tomsk
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