Identification of Clinical Isolates of  Bacillus cereus  Group and Their Characterization by Mass Spectrometry and Electron Microscopy

T. A. Smirnova; Смирнова Т. А.; N. B. Polyakov; Поляков Н. Б.; D. S. Karpov; Карпов Д. С.; A. I. Solovyev; Соловьев А. И.; N. V. Shevlyagina; Шевлягина Н. В.; S. G. Andreevskaya; Андреевская С. Г.; D. N. Shcherbinin; Щербинин Д. Н.; Z. S. Plieva; Плиева З. С.; V. A. Kozlova; Козлова В. А.; A. A. Pereborova; Переборова А. А.; I. A. Bogdanov; Богданов И. А.; D. A. Grumov; Грумов Д. А.; M. V. Zubasheva; Зубашева М. В.; S. V. Poddubko; Поддубко С. В.; A. A. Grechnikov; Гречников А. А.; M. A. Sukhina; Сухина М. А.; V. G. Zhukhovitsky; Жуховицкий В. Г.

doi:10.31857/S0026898423040201

Identification of Clinical Isolates of Bacillus cereus Group and Their Characterization by Mass Spectrometry and Electron Microscopy

Autores: Smirnova T.¹, Polyakov N.¹^,2, Karpov D.³, Solovyev A.¹, Shevlyagina N.¹, Andreevskaya S.¹, Shcherbinin D.¹, Plieva Z.¹, Kozlova V.¹, Pereborova A.¹, Bogdanov I.¹^,4, Grumov D.¹, Zubasheva M.¹, Poddubko S.⁵, Grechnikov A.², Sukhina M.⁶, Zhukhovitsky V.¹^,7
Afiliações:
1. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation
2. Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences
3. Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
4. “Nanopromimport” LLC, Moscow State University Science Park
5. State Scientific Center of the Russian Federation, Institute of Bio-Medical Problems, Russian Academy of Sciences
6. Ryzhikh State Research Center of Coloproctology, Ministry of Health
7. Russian Medical Academy of Continuing Professional Education (RMANPO), Ministry of Health of the Russian Federation
Edição: Volume 57, Nº 4 (2023)
Páginas: 609-622
Seção: ГЕНОМИКА. ТРАНСКРИПТОМИКА
URL: https://journals.rcsi.science/0026-8984/article/view/138696
DOI: https://doi.org/10.31857/S0026898423040201
EDN: https://elibrary.ru/QLYLNQ
ID: 138696

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Resumo

Bacillus cereus is a spore-forming bacterium found in the environment mainly in soil. Bacillus spores are known to be extremely resistant not only to environmental factors, but also to various sanitation regimes. This leads to spore contamination of toxin-producing strains in hospital and food equipment and, therefore, poses a great threat to human health. Two clinical isolates identified as B. cereus and B. cytotoxicus were investigated in the present work. It was shown that their calcium ion content was significantly lower than that of the reference strains. According to electron microscopy, one of the isolates SRCC 19/16 has an enlarged exosporium, and isolate SRCC 1208 shows large electron-dense inclusions of unclear nature during sporulation. We can assume that it contains a biologically active component with a cytotoxic effect and possibly plays a role in pathogenesis. Comparative chemical, biochemical, physiological, and ultrastructural analysis of spores of clinical isolates and reference strains of B. cereus was performed. The results obtained deepen our understanding of the properties of spores that contribute to the increased pathogenicity of B. cereus group species.

Palavras-chave

Bacillus cereus, Bacillus cytotoxicus, spore, electron microscopy, mass spectrometry

Bibliografia

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